FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1736281194> ?p ?o ?g. }

• W1736281194 endingPage "211" @default.
• W1736281194 startingPage "211" @default.
• W1736281194 abstract "In cattle, more than 30% of embryonic losses observed after artificial insemination (AI) have an early origin, coincident with a marked elongation of the trophoblast which occurs before implantation, between the 13th and 19th days of pregnancy. During this exponential growth phase, physiological interactions essential for pregnancy are established between the embryo and the uterus. Our work focuses on the identification of transcripts that regulate this key developmental period in several domestic species. For that, we generated a nylon membrane that contained 1920 gridded inserts originating from a Day-14 bovine embryo cDNA library (dbEST ID.15979; Hue et al., in preparation). Gene expression profiles in trophoblasts of increasing sizes were compared using ovoid (10–18-mm), tubular (50–60-mm), and early filamentous (140–150-mm) stages as complex probes. Trophoblasts were collected and immediately snap-frozen. RNA extractions were performed using RNAplus (Quantum Appligene, Illkirch 67402, France). Due to the scarce amount of mRNA per embryo, amplified material was used to hybridize the array. For that, antisense-RNA (aRNA) and cDNA were generated starting from 1 μg of total RNA, as described by the MessageAmp aRNA kit instructions (Ambion, Rusin, TX 78744, USA) and according to Revel et al. (1995 Zygote 3, 241–250). Five hundred nanograms of aRNA or cDNA were random-primed and labelled with 33P-alpha-dATP [aRNA, according to the procedure of Decraene et al. 1999 BioTechniques 27, 962–966; cDNA using the Atlas SMART Probe Amplification kit, (Clontech, Osyme, Saint Quentin Yvelines 78053, France)]. For each protocol, two probes were generated independently and each of these probes was hybridized to four identical membranes according to Clontech instructions. These were then exposed to phosphoscreens and scanned after 7 days. Quantifications were done using ImaGene 5.1 (BioDiscovery, El Segundo, CA 90245, USA) and statistically analyzed with the AnovArray package freely available for non-commercial use at http://www.jouy.inra.fr/stat/AnovArray (Piot et al. 2004 Bioinformatics, submitted). Reproducibility of the two protocols used to amplify material (aRNA and cDNA) was confirmed by slot blot quantifications before labelling. The hybridization profiles generated for each protocol (8 membranes per stage) were also highly reproducible (0.95 < r < 0.97), allowing a global statistical analysis with the AnovArray package. The results of the analysis of variance (ANOVA), including the correction for False Discovery Rate (FDR < 0.05), led to the identification of several bovine ESTs with unknown function that are differentially expressed during the rapid phase of trophoblastic elongation. Since genes, already known to be involved during elongation (IFN tau, Kunitz inhibitor), were also found differentially expressed in this study, this genomic approach using amplified complex probes is reliable to search for new markers of early developmental stages in cattle. Additionally, a thorough analysis of those markers may define them as interesting tools to assess the quality of embryonic development after AI, IVF (in vitro fertilisation), or SNT (somatic nuclear transfer). This work was supported by EU (BOI4-CT95-0190) and INRA-AGENA (AIPP00183) grants." @default.
• W1736281194 created "2016-06-24" @default.
• W1736281194 creator A5001237277 @default.
• W1736281194 creator A5065932349 @default.
• W1736281194 creator A5087232232 @default.
• W1736281194 date "2005-01-01" @default.
• W1736281194 modified "2023-10-14" @default.
• W1736281194 title "122 USE OF A DAY-14 EMBRYONIC ARRAY TO STUDY THE ELONGATION PHASE OF THE BOVINE EMBRYO" @default.
• W1736281194 doi "https://doi.org/10.1071/rdv17n2ab122" @default.
• W1736281194 hasPublicationYear "2005" @default.
• W1736281194 type Work @default.
• W1736281194 sameAs 1736281194 @default.
• W1736281194 citedByCount "0" @default.
• W1736281194 crossrefType "journal-article" @default.
• W1736281194 hasAuthorship W1736281194A5001237277 @default.
• W1736281194 hasAuthorship W1736281194A5065932349 @default.
• W1736281194 hasAuthorship W1736281194A5087232232 @default.
• W1736281194 hasBestOaLocation W17362811941 @default.
• W1736281194 hasConcept C104317684 @default.
• W1736281194 hasConcept C153911025 @default.
• W1736281194 hasConcept C172680121 @default.
• W1736281194 hasConcept C187882448 @default.
• W1736281194 hasConcept C196843134 @default.
• W1736281194 hasConcept C2776953305 @default.
• W1736281194 hasConcept C2777495146 @default.
• W1736281194 hasConcept C2779234561 @default.
• W1736281194 hasConcept C54355233 @default.
• W1736281194 hasConcept C86803240 @default.
• W1736281194 hasConcept C95444343 @default.
• W1736281194 hasConceptScore W1736281194C104317684 @default.
• W1736281194 hasConceptScore W1736281194C153911025 @default.
• W1736281194 hasConceptScore W1736281194C172680121 @default.
• W1736281194 hasConceptScore W1736281194C187882448 @default.
• W1736281194 hasConceptScore W1736281194C196843134 @default.
• W1736281194 hasConceptScore W1736281194C2776953305 @default.
• W1736281194 hasConceptScore W1736281194C2777495146 @default.
• W1736281194 hasConceptScore W1736281194C2779234561 @default.
• W1736281194 hasConceptScore W1736281194C54355233 @default.
• W1736281194 hasConceptScore W1736281194C86803240 @default.
• W1736281194 hasConceptScore W1736281194C95444343 @default.
• W1736281194 hasIssue "2" @default.
• W1736281194 hasLocation W17362811941 @default.
• W1736281194 hasOpenAccess W1736281194 @default.
• W1736281194 hasPrimaryLocation W17362811941 @default.
• W1736281194 hasRelatedWork W1892544028 @default.
• W1736281194 hasRelatedWork W2048908210 @default.
• W1736281194 hasRelatedWork W2089006500 @default.
• W1736281194 hasRelatedWork W2105450271 @default.
• W1736281194 hasRelatedWork W2138547054 @default.
• W1736281194 hasRelatedWork W2169340257 @default.
• W1736281194 hasRelatedWork W2430529198 @default.
• W1736281194 hasRelatedWork W2509789438 @default.
• W1736281194 hasRelatedWork W2596000028 @default.
• W1736281194 hasRelatedWork W4283014574 @default.
• W1736281194 hasVolume "17" @default.
• W1736281194 isParatext "false" @default.
• W1736281194 isRetracted "false" @default.
• W1736281194 magId "1736281194" @default.
• W1736281194 workType "article" @default.