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- W1736394754 abstract "As avidin combines stoichiometrically with biotin, it is possible to use any physicochemical difference between avidin and the avidin–biotin complex as the basis of an assay method for either component. Two convenient techniques are described in this chapter. The first is based on the red shift of the absorption spectrum of the tryptophan residues of avidin, which accompanies combination with biotin, and requires measurement of the change in optical density at 233nm. The second is based on the use of the dye 4-hydroxyazobenzene-2′-carboxylic acid (HABA), which binds only to avidin and can, therefore, be used as an indicator for unoccupied binding sites. The method based on spectral shift is technically more demanding and has no advantages over the dye-binding method for routine estimations, but it is useful for the study of biotin analogs. Both methods are of lower sensitivity than the bioassays, but they are more precise, more convenient, and can be used over a wide range of pH and salt concentration." @default.
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- W1736394754 date "1970-01-01" @default.
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- W1736394754 title "[74] Spectrophotometric determination of avidin and biotin" @default.
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- W1736394754 doi "https://doi.org/10.1016/0076-6879(71)18342-5" @default.
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