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- W173655547 abstract "The work described in this thesis involved the production of antibodies to several defined antigens and the chemical synthesis of bispecific antibodies.Monoclonal antibodies against glial fibrillary acidic protein (anti-GFAP), and against a GFAP-like proteinon the human anaplastic astrocytoma cell line, G-CCM (anti-G-CCM) were produced by electrofusion and by fusion using polyethylene glycol, respectively. These antibodies were purified with saturated ammonium sulphate (S AS) and protein A. These antibodies were fully characterised by ELISA, Isotyping, SDS-PAGE, Immunocylochemisiry, Dot Blot, Western immunoblotting, BIAcore, HPLC, Immunofluoresence and FACS An attempt was made to clone and express the variable regions of the anti-G-CCM monoclonal antibody in Escherichia colt.Iodinated Bolton-Hunter reagent (IBHR) was conjugated to bovine serum albumin (BSA), ovalbumin and keyhole limpet haemocyanin (KLH). These IBHR-protein conjugates were characterised by the iodide assay and the Bradford protein assay. The IBHR-BSA conjugate was used to immunise six mice and a rabbit to produce anti-IBHR antibodies. A monoclonal anti-IBHR antibody was not obtained. However,a polyclonal anti-IBHR antibody was produced and was purified by SAS, protein A and CNBr-activatedsepharose affinity chromatography. This antibody was characterised by ELISA, SDS-PAGE and HPLC Bispecific antibodies were chemically generated using (1) anti-GFAP and ana-IBHR and (u) anti-G-CCM and anti-IBHR Ricin is a highly cytotoxic compound Bispecific antibodies were made using an acquired anti-Ricm. A chain monoclonal antibody together with anti-G-CCM and anti-GFAP. These bispecific antibodies were characterised by ELISA, SDS-PAGE, HPLC and FACS and may be used to target radioactive compounds and toxins to astrocytoma cells, in vivo.A catalytic monoclonal antibody was generated using a transition state analogue-KLH conjugate as an immunogen in six mice. This antibody was precipitated using sodium sulphate and purified by protein G affinity chromatography. This antibody catalysed the hydrolysis of the corresponding carbonate and its rate kinetics were determined." @default.
- W173655547 created "2016-06-24" @default.
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- W173655547 date "1994-01-01" @default.
- W173655547 modified "2023-09-27" @default.
- W173655547 title "Studies on the production, characterisation and applications of monoclonal antibodies and antibody-based conjugates" @default.
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