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- W1736678239 abstract "Analysis of mtDNA HVSI/II region for forensic identification had been widely validated although there is chance to find identical HVSI/II between individuals in random. Examination of SNPs in the entire mitochondrial genome also had been proposed to increase the specificity of examination. Here we show, a simple way to increase the resolution of identification, by discriminating individuals sharing identical HVSI/II using one additional nucleotide analysis only, that is the ATPase6 gene segment (nt 8553-8903). We examined 542 mtDNA complete genome data from three main population groups obtained from public sites. Additionally, nucleotide sequences of the most diverse segment were obtained from 30 Indonesian individuals as primary data. The discriminating power was determined by counting the number of individuals that could be discriminated by nucleotide analysis. The ATPase6 gene segment consistently demonstrates higher value of haplotype diversity compared to the other segments excluding HVSI/II, up to 0.955, 0.954, 0.855 in the African (n=40), Asian (n=95) and Caucasian (n=407) population groups respectively. In the Indonesian population group the diversity reached 0.855. The examination of ATPase6 gene segment increased the number of haplotypes compared to the use of HVSI/II analysis only, from 51 to 87. In the Caucasian population group only 182 individuals were discriminated by HVSI/II analysis only, and the SNPs clustered in the ATPase6 gene segment increased the number of individuals up to 218. ATPase6 gene segment can be suggested as additional sequencing target of analysis to discriminate individuals or biological samples sharing identical HVSI/II, particularly when overall conclusion is in doubt. Keywords : mtDNA, ATPase6, forensic identification, mitochondrial genome" @default.
- W1736678239 created "2016-06-24" @default.
- W1736678239 creator A5001365181 @default.
- W1736678239 date "2011-03-31" @default.
- W1736678239 modified "2023-09-26" @default.
- W1736678239 title "The Variability of Human mtDNA ATPase6 Gene Segment (nt 8553-8903) for Augmenting Forensic Identification" @default.
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