Matches in SemOpenAlex for { <https://semopenalex.org/work/W1738340030> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W1738340030 endingPage "1069" @default.
- W1738340030 startingPage "1061" @default.
- W1738340030 abstract "The interaction of β-trypsin [EC 3.4.21.4] immobilized on Sepharose with competitive inhibitors was quantitatively studied by frontal affinity chromatography. Analysis of the dependence of the elution volume of an inhibitor on its initial concentration gave two important parameters. One is the dissociation constant (Kd) of the immobilized trypsin-inhibitor complex, and the other is the total amount of trypsin retaining binding ability that is present in the column (Bt). Experiments using benzamidine showed that frontal affinity chromatography is a theoretically simple system and can be treated in the same way as enzyme kinetics. The Kd value obtained for benzamidine was very similar to the K1 value. The Bt value corresponded to 73% of the immobilized trypsin. These results suggest that the function of trypsin was hardly affected by the immobilization. Results obtained with β-naphthamidine were somewhat less satisfactory, probably due to a considerable non-specific interaction. In the case of proflavine, analysis was almost impossible due to extremely strong non-specific interaction. This procedure is very useful to analyze specific interactions if nonspecific interactions are not significant. The basic features of frontal affinity chromatography are discussed." @default.
- W1738340030 created "2016-06-24" @default.
- W1738340030 creator A5014756582 @default.
- W1738340030 creator A5078683191 @default.
- W1738340030 date "1978-11-01" @default.
- W1738340030 modified "2023-09-23" @default.
- W1738340030 title "Studies on the Interaction of Immobilized Trypsin and Specific Ligands by Quantitative Affinity Chromatography" @default.
- W1738340030 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a132220" @default.
- W1738340030 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/730749" @default.
- W1738340030 hasPublicationYear "1978" @default.
- W1738340030 type Work @default.
- W1738340030 sameAs 1738340030 @default.
- W1738340030 citedByCount "36" @default.
- W1738340030 countsByYear W17383400302012 @default.
- W1738340030 countsByYear W17383400302013 @default.
- W1738340030 countsByYear W17383400302014 @default.
- W1738340030 countsByYear W17383400302020 @default.
- W1738340030 countsByYear W17383400302021 @default.
- W1738340030 crossrefType "journal-article" @default.
- W1738340030 hasAuthorship W1738340030A5014756582 @default.
- W1738340030 hasAuthorship W1738340030A5078683191 @default.
- W1738340030 hasConcept C170493617 @default.
- W1738340030 hasConcept C179247698 @default.
- W1738340030 hasConcept C181199279 @default.
- W1738340030 hasConcept C185592680 @default.
- W1738340030 hasConcept C206967267 @default.
- W1738340030 hasConcept C2779288520 @default.
- W1738340030 hasConcept C2779881004 @default.
- W1738340030 hasConcept C2780340462 @default.
- W1738340030 hasConcept C43617362 @default.
- W1738340030 hasConcept C55493867 @default.
- W1738340030 hasConcept C73826308 @default.
- W1738340030 hasConcept C74998103 @default.
- W1738340030 hasConceptScore W1738340030C170493617 @default.
- W1738340030 hasConceptScore W1738340030C179247698 @default.
- W1738340030 hasConceptScore W1738340030C181199279 @default.
- W1738340030 hasConceptScore W1738340030C185592680 @default.
- W1738340030 hasConceptScore W1738340030C206967267 @default.
- W1738340030 hasConceptScore W1738340030C2779288520 @default.
- W1738340030 hasConceptScore W1738340030C2779881004 @default.
- W1738340030 hasConceptScore W1738340030C2780340462 @default.
- W1738340030 hasConceptScore W1738340030C43617362 @default.
- W1738340030 hasConceptScore W1738340030C55493867 @default.
- W1738340030 hasConceptScore W1738340030C73826308 @default.
- W1738340030 hasConceptScore W1738340030C74998103 @default.
- W1738340030 hasIssue "5" @default.
- W1738340030 hasLocation W17383400301 @default.
- W1738340030 hasLocation W17383400302 @default.
- W1738340030 hasOpenAccess W1738340030 @default.
- W1738340030 hasPrimaryLocation W17383400301 @default.
- W1738340030 hasRelatedWork W1738340030 @default.
- W1738340030 hasRelatedWork W2025421340 @default.
- W1738340030 hasRelatedWork W2053774504 @default.
- W1738340030 hasRelatedWork W2056193646 @default.
- W1738340030 hasRelatedWork W2068841117 @default.
- W1738340030 hasRelatedWork W2072577350 @default.
- W1738340030 hasRelatedWork W2076720835 @default.
- W1738340030 hasRelatedWork W2087550423 @default.
- W1738340030 hasRelatedWork W2388013284 @default.
- W1738340030 hasRelatedWork W572440283 @default.
- W1738340030 hasVolume "84" @default.
- W1738340030 isParatext "false" @default.
- W1738340030 isRetracted "false" @default.
- W1738340030 magId "1738340030" @default.
- W1738340030 workType "article" @default.