Matches in SemOpenAlex for { <https://semopenalex.org/work/W1738721594> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W1738721594 endingPage "445" @default.
- W1738721594 startingPage "435" @default.
- W1738721594 abstract "A new in vitro system for T4 DNA replication was developed by concentrating cell lysates on cellophane disks. The time course of [3H]dTTP incorporation into DNA by the system was separated into two phases: one was a very rapid incorporation which was terminated within 2 min (phase I reaction), and the other was a slow but continuous incorporation thereafter (phase II reaction). More than half of the phase I reaction product was Escherichia coli DNA, but the phase II reaction was mostly T4 DNA. Phase II reaction required four deoxyribonucleoside triphosphates, ATP, Mg2+, and KCl. 5-Hydroxymethyldeoxycytidine triphosphate was essential for the reaction and not substitutable by dCTP. The presence of KCN or NaN3 in the reaction mixture did not interfere with [3H]dTTP incorporation, but the addition of deoxyribonuclease completely degraded the system. Alkaline sucrose sedimentation analysis of phage II reaction product revealed that phase II reaction proceeded by the discontinuous mode of DNA replication as in vivo. After T4 infection, the activity for phase II reaction appeared in parallel with the activity of T4 phage DNA replication in vivo." @default.
- W1738721594 created "2016-06-24" @default.
- W1738721594 creator A5022188566 @default.
- W1738721594 creator A5031408829 @default.
- W1738721594 date "1976-08-01" @default.
- W1738721594 modified "2023-09-25" @default.
- W1738721594 title "Replication of bacteriophage T4 DNA in vitro. I. Basic properties of the system" @default.
- W1738721594 cites W1554385615 @default.
- W1738721594 cites W1554753165 @default.
- W1738721594 cites W1817599094 @default.
- W1738721594 cites W1833575129 @default.
- W1738721594 cites W1966559144 @default.
- W1738721594 cites W1970871621 @default.
- W1738721594 cites W1984392393 @default.
- W1738721594 cites W2007011423 @default.
- W1738721594 cites W2013656249 @default.
- W1738721594 cites W2015987789 @default.
- W1738721594 cites W2028082957 @default.
- W1738721594 cites W2037427057 @default.
- W1738721594 cites W2055491259 @default.
- W1738721594 cites W2057576911 @default.
- W1738721594 cites W2078872641 @default.
- W1738721594 cites W2079367762 @default.
- W1738721594 cites W2086511883 @default.
- W1738721594 cites W2093855124 @default.
- W1738721594 cites W2131507346 @default.
- W1738721594 cites W2215937809 @default.
- W1738721594 cites W2331676146 @default.
- W1738721594 cites W2495373767 @default.
- W1738721594 cites W63285388 @default.
- W1738721594 doi "https://doi.org/10.1128/jvi.19.2.435-445.1976" @default.
- W1738721594 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/354881" @default.
- W1738721594 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/785023" @default.
- W1738721594 hasPublicationYear "1976" @default.
- W1738721594 type Work @default.
- W1738721594 sameAs 1738721594 @default.
- W1738721594 citedByCount "7" @default.
- W1738721594 crossrefType "journal-article" @default.
- W1738721594 hasAuthorship W1738721594A5022188566 @default.
- W1738721594 hasAuthorship W1738721594A5031408829 @default.
- W1738721594 hasBestOaLocation W17387215941 @default.
- W1738721594 hasConcept C104317684 @default.
- W1738721594 hasConcept C153911025 @default.
- W1738721594 hasConcept C202751555 @default.
- W1738721594 hasConcept C2776441376 @default.
- W1738721594 hasConcept C2778659741 @default.
- W1738721594 hasConcept C547475151 @default.
- W1738721594 hasConcept C552990157 @default.
- W1738721594 hasConcept C55493867 @default.
- W1738721594 hasConcept C64241487 @default.
- W1738721594 hasConcept C73573662 @default.
- W1738721594 hasConcept C86803240 @default.
- W1738721594 hasConceptScore W1738721594C104317684 @default.
- W1738721594 hasConceptScore W1738721594C153911025 @default.
- W1738721594 hasConceptScore W1738721594C202751555 @default.
- W1738721594 hasConceptScore W1738721594C2776441376 @default.
- W1738721594 hasConceptScore W1738721594C2778659741 @default.
- W1738721594 hasConceptScore W1738721594C547475151 @default.
- W1738721594 hasConceptScore W1738721594C552990157 @default.
- W1738721594 hasConceptScore W1738721594C55493867 @default.
- W1738721594 hasConceptScore W1738721594C64241487 @default.
- W1738721594 hasConceptScore W1738721594C73573662 @default.
- W1738721594 hasConceptScore W1738721594C86803240 @default.
- W1738721594 hasIssue "2" @default.
- W1738721594 hasLocation W17387215941 @default.
- W1738721594 hasLocation W17387215942 @default.
- W1738721594 hasLocation W17387215943 @default.
- W1738721594 hasOpenAccess W1738721594 @default.
- W1738721594 hasPrimaryLocation W17387215941 @default.
- W1738721594 hasRelatedWork W1482484754 @default.
- W1738721594 hasRelatedWork W1507344158 @default.
- W1738721594 hasRelatedWork W1528843118 @default.
- W1738721594 hasRelatedWork W1578931006 @default.
- W1738721594 hasRelatedWork W1608199527 @default.
- W1738721594 hasRelatedWork W1787424949 @default.
- W1738721594 hasRelatedWork W1980215918 @default.
- W1738721594 hasRelatedWork W2084555268 @default.
- W1738721594 hasRelatedWork W2119283398 @default.
- W1738721594 hasRelatedWork W2167165547 @default.
- W1738721594 hasVolume "19" @default.
- W1738721594 isParatext "false" @default.
- W1738721594 isRetracted "false" @default.
- W1738721594 magId "1738721594" @default.
- W1738721594 workType "article" @default.