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• W1738796567 endingPage "3164" @default.
• W1738796567 startingPage "3159" @default.
• W1738796567 abstract "Triple gene block (TGB), a conserved gene module found in the genomes of many filamentous and rod-shaped plant viruses, encodes three proteins, TGB1, TGB2 and TGB3, required for viral cell-to-cell movement through plasmodesmata and systemic transport via the phloem. The genome of Shallot virus X, the type species of the genus Allexivirus, includes TGB1 and TGB2 genes, but contains no canonical ORF for TGB3 protein. However, a TGB3-like protein-encoding sequence lacking an AUG initiator codon has been found in the shallot virus X (ShVX) genome in a position typical for TGB3 genes. This putative TGB3 gene is conserved in all allexiviruses. Here, we carried out sequence analysis to predict possible non-AUG initiator codons in the ShVX TGB3-encoding sequence. We further used an agroinfiltration assay in Nicotiana benthamiana to confirm this prediction. Site-directed mutagenesis was used to demonstrate that the ShVX TGB3 could be translated on a bicistronic mRNA template via a leaky scanning mechanism." @default.
• W1738796567 created "2016-06-24" @default.
• W1738796567 creator A5006811016 @default.
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• W1738796567 creator A5086166941 @default.
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• W1738796567 date "2015-10-01" @default.
• W1738796567 modified "2023-10-03" @default.
• W1738796567 title "Translation of the shallot virus X TGB3 gene depends on non-AUG initiation and leaky scanning" @default.
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• W1738796567 doi "https://doi.org/10.1099/jgv.0.000248" @default.
• W1738796567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26296665" @default.
• W1738796567 hasPublicationYear "2015" @default.
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