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• W1738796572 startingPage "264" @default.
• W1738796572 abstract "Monitoring transcription factor (TF) levels provides an important assessment of the state of cell populations. Unfortunately, traditional methods for monitoring TF concentration are generally cumbersome and time-consuming. We developed an ultrasensitive one-pot TF detection method that uses target-molecular beacons-dependent amplification (TMDA) fluorescence strategy to circumvent the aforementioned limitations in TF detection. In this assay, we employed a DNA1/DNA2 duplex as the reporting probe and a stem-loop DNA molecular beacon (MB) as the signaling probe. The integration of protein-DNA1/DNA2 duplex and exonuclease III (Exo III) digestion can convert the detection of transcription factors to the detection of reporter oligonucleotides. The subsequent hybridization of the reporter oligonucleotides with the molecular beacons opens the stem-loop structure. The formation of the DNA complex triggers amplification reaction and the recovery of the fluorescence. This assay exhibits high sensitivity with a detection limit of 2.2 pM and a detection range of 3 orders of magnitude, which is superior to most currently used methods for transcription factor detection. More importantly, this method is suitable for the direct detection of TFs in crude nuclear extracts of cancer cells." @default.
• W1738796572 created "2016-06-24" @default.
• W1738796572 creator A5032799600 @default.
• W1738796572 creator A5061190026 @default.
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• W1738796572 date "2016-03-01" @default.
• W1738796572 modified "2023-10-13" @default.
• W1738796572 title "Sensitive detection of transcription factors in cell nuclear extracts by using a molecular beacons based amplification strategy" @default.
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• W1738796572 doi "https://doi.org/10.1016/j.bios.2015.09.040" @default.
• W1738796572 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26410390" @default.
• W1738796572 hasPublicationYear "2016" @default.
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