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• W1741145107 abstract "ABSTRACT In order to devise an in vivo insertion mutagenesis scheme for Haemophilus influenzae , a novel set of transposons has been constructed. These are Tn 10 -based minitransposons carried on pACYC184- and pACYC177-based replicons, which are suitable for in vivo transposition in H. influenzae . The transposon delivery system was designed to contain an H. influenzae -specific uptake signal sequence which facilitates DNA transformation into H. influenzae . The following mini-Tn 10 elements have been made suitable for specific tasks in H. influenzae : (i) Tn 10d-cat , which can be used to generate chloramphenicol-selectable insertion mutations; (ii) Tn 10d-bla , an ampicillin-selectable translational fusion system allowing the detection of membrane or secreted proteins; and (iii) Tn 10d-lacZcat , a chloramphenicol-selectable lacZ transcriptional fusion system. For the rapid identification of the transposon insertions, a PCR fragment enrichment method was developed. This report demonstrates that this in vivo mutagenesis technique is a convenient tool for the analysis of biochemical and regulatory pathways in the human pathogen H. influenzae ." @default.
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• W1741145107 date "1998-12-01" @default.
• W1741145107 modified "2023-09-23" @default.
• W1741145107 title "In Vivo Transposon Mutagenesis in Haemophilus influenzae" @default.
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• W1741145107 doi "https://doi.org/10.1128/aem.64.12.4697-4702.1998" @default.
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