FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1745021804> ?p ?o ?g. }

• W1745021804 endingPage "8101" @default.
• W1745021804 startingPage "8094" @default.
• W1745021804 abstract "We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yielding an apparent K0.5 for Hg(II)-stimulated induction by MerR of 9.3 x 10(-8) M with the same ultrasensitive threshold effect seen in vitro. We also used the mer-lux fusions to elucidate subtle variations in promoter activity brought about by altered superhelicity. Binding of inducer [Hg(II)] to the transcriptional activator MerR is known to result in DNA distortion and transcriptional activation of the mer operon; it has recently been demonstrated that this distortion is a consequence of MerR-Hg(II)-induced local DNA unwinding to facilitate RNA polymerase open complex formation at PTPCAD. Since negative supercoiling results in DNA unwinding similar to this MerR activation, we hypothesized that a global increase in plasmid supercoiling would facilitate MerR-mediated activation and compromise MerR-mediated repression, while removal of plasmid supercoils would compromise MerR's ability to induce transcription and facilitate its ability to repress transcription. Indeed, we found that increased negative supercoiling results in increased gene expression rates and decreased supercoiling results in reduced gene expression rates for the induced, repressed, and derepressed conditions of PTPCAD. Thus, luciferase transcriptional fusions can detect subtle variations in initial rates of gene expression in a real-time, nondestructive assay." @default.
• W1745021804 created "2016-06-24" @default.
• W1745021804 creator A5021047697 @default.
• W1745021804 creator A5087737425 @default.
• W1745021804 date "1992-12-01" @default.
• W1745021804 modified "2023-10-15" @default.
• W1745021804 title "A mer-lux transcriptional fusion for real-time examination of in vivo gene expression kinetics and promoter response to altered superhelicity" @default.
• W1745021804 cites W1561314350 @default.
• W1745021804 cites W1592215057 @default.
• W1745021804 cites W1664318623 @default.
• W1745021804 cites W1684235888 @default.
• W1745021804 cites W1830190901 @default.
• W1745021804 cites W1847952945 @default.
• W1745021804 cites W1902476841 @default.
• W1745021804 cites W1919756885 @default.
• W1745021804 cites W1944444828 @default.
• W1745021804 cites W1969511411 @default.
• W1745021804 cites W1969979186 @default.
• W1745021804 cites W1970585122 @default.
• W1745021804 cites W1971670546 @default.
• W1745021804 cites W1974552460 @default.
• W1745021804 cites W1989493141 @default.
• W1745021804 cites W1999570631 @default.
• W1745021804 cites W2001205739 @default.
• W1745021804 cites W2005392755 @default.
• W1745021804 cites W2005487874 @default.
• W1745021804 cites W2019410656 @default.
• W1745021804 cites W2020954737 @default.
• W1745021804 cites W2022571490 @default.
• W1745021804 cites W2032441027 @default.
• W1745021804 cites W2040654367 @default.
• W1745021804 cites W2041603328 @default.
• W1745021804 cites W2042400188 @default.
• W1745021804 cites W2046392322 @default.
• W1745021804 cites W2046850778 @default.
• W1745021804 cites W2052924273 @default.
• W1745021804 cites W2056406408 @default.
• W1745021804 cites W2058940094 @default.
• W1745021804 cites W2060384825 @default.
• W1745021804 cites W2079123741 @default.
• W1745021804 cites W2080131435 @default.
• W1745021804 cites W2084566718 @default.
• W1745021804 cites W2089767383 @default.
• W1745021804 cites W2090486130 @default.
• W1745021804 cites W2119076989 @default.
• W1745021804 cites W2140158396 @default.
• W1745021804 cites W2147866874 @default.
• W1745021804 doi "https://doi.org/10.1128/jb.174.24.8094-8101.1992" @default.
• W1745021804 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/207548" @default.
• W1745021804 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1334070" @default.
• W1745021804 hasPublicationYear "1992" @default.
• W1745021804 type Work @default.
• W1745021804 sameAs 1745021804 @default.
• W1745021804 citedByCount "88" @default.
• W1745021804 countsByYear W17450218042012 @default.
• W1745021804 countsByYear W17450218042014 @default.
• W1745021804 countsByYear W17450218042015 @default.
• W1745021804 countsByYear W17450218042016 @default.
• W1745021804 countsByYear W17450218042017 @default.
• W1745021804 countsByYear W17450218042019 @default.
• W1745021804 countsByYear W17450218042020 @default.
• W1745021804 countsByYear W17450218042021 @default.
• W1745021804 countsByYear W17450218042022 @default.
• W1745021804 countsByYear W17450218042023 @default.
• W1745021804 crossrefType "journal-article" @default.
• W1745021804 hasAuthorship W1745021804A5021047697 @default.
• W1745021804 hasAuthorship W1745021804A5087737425 @default.
• W1745021804 hasBestOaLocation W17450218041 @default.
• W1745021804 hasConcept C104317684 @default.
• W1745021804 hasConcept C111335760 @default.
• W1745021804 hasConcept C111829193 @default.
• W1745021804 hasConcept C138885662 @default.
• W1745021804 hasConcept C143065580 @default.
• W1745021804 hasConcept C150194340 @default.
• W1745021804 hasConcept C153911025 @default.
• W1745021804 hasConcept C158456008 @default.
• W1745021804 hasConcept C165864922 @default.
• W1745021804 hasConcept C179926584 @default.
• W1745021804 hasConcept C203075996 @default.
• W1745021804 hasConcept C22744801 @default.
• W1745021804 hasConcept C27153228 @default.
• W1745021804 hasConcept C2776449523 @default.
• W1745021804 hasConcept C41895202 @default.
• W1745021804 hasConcept C54009773 @default.
• W1745021804 hasConcept C54355233 @default.
• W1745021804 hasConcept C552990157 @default.
• W1745021804 hasConcept C67705224 @default.
• W1745021804 hasConcept C73573662 @default.
• W1745021804 hasConcept C86803240 @default.
• W1745021804 hasConcept C88045685 @default.
• W1745021804 hasConcept C95444343 @default.
• W1745021804 hasConceptScore W1745021804C104317684 @default.
• W1745021804 hasConceptScore W1745021804C111335760 @default.
• W1745021804 hasConceptScore W1745021804C111829193 @default.
• W1745021804 hasConceptScore W1745021804C138885662 @default.
• W1745021804 hasConceptScore W1745021804C143065580 @default.
• W1745021804 hasConceptScore W1745021804C150194340 @default.
• W1745021804 hasConceptScore W1745021804C153911025 @default.

• next