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• W1746652246 abstract "Objective: To assess the potential of vitrification of human immature testicular tissue in an in vivo xenotransplantation model and compare it with slow-freezing and fresh tissue xenografting. Material and Methods: Single 1 mm³ pieces of immature testicular tissue were obtained from 7 prepubertal boys (2–12 years). Fragments of fresh tissue (serving as controls) and fresh, frozen-thawed and vitrified-warmed testicular pieces xenografted to the scrotum of nude mice for 6 months were compared. Tissue was vitrified using basic Leibovitz L-15 medium supplemented with 25 mg/mL human serum albumin containing dimethyl sulfoxide 15%, ethylene glycol 15% and 0.5M sucrose as cryoprotectants. For controlled slow-freezing, dimethyl sulfoxide 5% was supplemented with 0.1M sucrose and 10 mg/ml human serum albumin as cryoprotectants. Upon removal, histological and immunohistochemical analyses were performed to evaluate the tissue. The totality of the grafts was assessed. Results: Histology showed 89.14%, 86.39% and 89.73% of seminiferous tubules to be intact in fresh, frozen-thawed and vitrified tissue grafts respectively. MAGE-A4 immunostaining showed mean spermatogonial recovery to be 3.20.3%, 0.830.05% and 1.380.21% respectively, not significantly different between the three groups. Moreover, 13.920.21%, 12.990.11% and 9.350.11% of these cells expressed Ki67, evidencing proliferation of spermatogonia in transplanted tissue, compared to 0.50.01% in fresh non-grafted control tissue. A few spermatocytes at the pachytene stage were encountered in slow-frozen and vitrified grafts from one 9-year-old boy, while this differentiation stage was not observed in the others. Conclusion: Vitrification was found to preserve the integrity of seminiferous tubules in human immature testicular tissue and maintain survival and proliferation of spermatogonia after long-term transplantation. A similar decrease in spermatogonial number was observed in fresh, frozen-thawed and vitrified-warmed grafts, suggesting that the grafting procedure, and not only the freezing procedure, may be involved. Although vitrification appears to be a promising strategy for fertility preservation, further studies should be conducted to evaluate spermatogonial differentiation in long-term grafts before the technique may be validated." @default.
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• W1746652246 date "2011-01-01" @default.
• W1746652246 modified "2023-09-27" @default.
• W1746652246 title "Vitrification of human immature testicular tissue allows maintainance of proliferatingspermatogonial cells after xenografting to recipient mice" @default.
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