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- W1747071196 abstract "Microbial laccases are powerful enzymes capable of degrading lignin and other recalcitrant compounds. For that reason, there is an increasing interest in these enzymes as biocatalysts for the removal of micropollutants in wastewater, with very promising results so far. However, the establishment of efficient industrial processes greatly depends on the availability of robust, stable and cost-effective immobilized biocatalysts. Recently, a novel immobilization technique based on bio-inspired enzyme entrapment has emerged as a fast and versatile tool for generating robust, cheap and highly stable (nano)biocatalysts, with a very high potential for environmental applications. The enzyme entrapment via bio-inspired mineralization aims to mimic the natural reactions in diatoms, whereby silica precipitation is induced by polypeptides called silaffins. By analogy, different polymers (natural or synthetic) are able to induce the precipitation of different inorganic oxides to form different nanosized structures in which the enzyme is entrapped. In a proof-of-concept experiment, biocatalysts with 25 U/g were produced using polyethylenimine as the synthetic polypeptide analogue inducing the precipitation of biotitania on the surface of magnetic particles to allow an easy recovery of the immobilized biocatalysts with an external magnetic field. Different immobilization parameters (enzyme loading, titania precursor concentration, pH, reaction and laccase adsorption times) were varied in order to identify the key phenomena controlling the enzyme entrapment and improve the activity of the biotitania-laccase particles. Almost 10-times higher activity loading (up to 200 U/g) was obtained after optimization of the immobilization parameters. The biocatalytic potential of the biotitania-laccase particles with magnetic properties was assessed for the degradation of a mixture of model micropollutants in wastewater: sulfamethoxazole (antibiotic), bisphenol A (plasticizer), 17α-ethinylestradiol (synthetic estrogen), diclofenac (anti-inflammatory), triclosan (biocide) and nonylphenol (detergent by-product). Degradation experiments in batch showed that the biocatalytic capability of the laccase for the oxidation of these compounds was preserved after immobilization. Additionally, a 100 mL continuous reactor prototype with magnetic particle retention was set up to test the degradation at laboratory scale of the mixture of 6 model micropollutants, obtaining degradation efficiencies of 60% for 17α-ethynylestradiol, 45% of bisphenol A and 30% for diclofenac." @default.
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- W1747071196 date "2013-01-01" @default.
- W1747071196 modified "2023-09-27" @default.
- W1747071196 title "Laccase-biotitania magnetic biocatalysts produced via bio-inspired entrapment for the removal of micropollutants in wastewater" @default.
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