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• W1748656482 abstract "Abstract Breast reconstruction surgeries using tissue expanders (TEs) have highly reported infection rates. To decrease this, we developed a method for disinfecting TEs and surgical pockets, where an antimicrobial solution was applied as a solid film at implantation that subsequently liquefied in situ to provide extended prophylaxis. Silicone discs cut from TEs were covered with gelatin‐based films containing minocycline (M) and rifampin (R). Discs and films soaked in saline were subsequently challenged with pathogen at days 1, 3, 7, and 10 and quantified for potential biofilm formation. Discs that were not harvested at each specific time points were refreshed with sterile saline. The discs were challenged with clinical isolates of methicillin‐resistant Staphylococcus aureus (MRSA), methicillin‐resistant Staphylococcus epidermidis (MRSE), and multidrug‐resistant Pseudomonas aeruginosa (MDR‐PA). Recoveries of adherent organisms from uncovered silicone discs and gelatin‐wrapped discs without added antimicrobial agents were >5 × 10 4 CFU/disc for each organism at each time point. Experimental 0.1%M/0.05%R gelatin films completely inhibited all challenge organisms from attaching to the silicone ( p < 0.05) at each time point through day 10. Cytotoxicity was assessed by incubating films with HEK‐293T human fibroblasts. There were no significant differences in HEK‐293T cell survival between controls and any of the antimicrobial films. The in situ liquefying, bioabsorable, antimicrobial wrap prevented biofilm formation by microorganisms on silicone surfaces in vitro with minimal cytotoxicity. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 369–374, 2016." @default.
• W1748656482 created "2016-06-24" @default.
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• W1748656482 date "2015-03-23" @default.
• W1748656482 modified "2023-10-18" @default.
• W1748656482 title "Novel<i>in situ</i>liquefying antimicrobial wrap for preventing tissue expander infections following breast reconstructive surgeries" @default.
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• W1748656482 doi "https://doi.org/10.1002/jbm.b.33399" @default.
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