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• W1749321702 endingPage "2444" @default.
• W1749321702 startingPage "2431" @default.
• W1749321702 abstract "Fragments from the Ty-D15 element of Saccharomyces cerevisiae were assayed for the ability to direct 3'-end formation for RNA initiated by the GAL1 promoter. The delta, the direct repeat at each end of the element, was capable of forming 3' ends at two sites, an inefficient upstream site and an efficient downstream site near the end of the delta. Different sequences were required for 3'-end formation at these sites. For the efficient site, all transcripts had 3' ends in the delta and no downstream transcription was detected, which suggested that these sequences terminate transcription. Surprisingly, the delta region downstream of the initiation site for Ty RNA comprised part of this major site and terminated more than 50% of the transcripts that read into it. Sequences necessary for the efficient site were localized to two small regions. Both regions were upstream of the 3' end and contained similarities to a tripartite consensus sequence that has been proposed as a terminator element. Sequences near the position of the 3' end could also affect termination; a short G + C-rich sequence inserted just downstream changed an efficient terminator to an inefficient one. Initiation in the delta had no effect on the efficiency or positions or termination in that delta. A new initiation site was seen when the same delta terminated transcription, but transcriptional interference did not occur, since the amount of initiation was not decreased." @default.
• W1749321702 created "2016-06-24" @default.
• W1749321702 creator A5018909353 @default.
• W1749321702 creator A5064158091 @default.
• W1749321702 date "1989-06-01" @default.
• W1749321702 modified "2023-10-17" @default.
• W1749321702 title "Some of the signals for 3'-end formation in transcription of the Saccharomyces cerevisiae Ty-D15 element are immediately downstream of the initiation site" @default.
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• W1749321702 doi "https://doi.org/10.1128/mcb.9.6.2431-2444.1989" @default.
• W1749321702 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/362316" @default.
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