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- W1749586744 abstract "Publisher Summary This chapter describes procedure for the assay of hydration of dehydro- L -ascorbic acid and purification and assay of decarboxylation of 2,3-diketo- L -gulonic acid. The purification procedure of decarboxylation of 2,3-diketo- L -gulonic acid involves three steps—namely, the preparation of supernatant fraction, ammonium sulfate precipitation, and cellulose column chromatography. Diketo- L -gulonic acid lost and CO 2 produced are almost proportional to the incubation time. The chapter discusses the properties of 2,3-diketo- L -gulonic acid that involve the effect of pH, stability of enzyme, activators and inhibitors, substrate concentration, substrate specificity, and distribution of decarboxylase." @default.
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- W1749586744 date "1970-01-01" @default.
- W1749586744 modified "2023-09-27" @default.
- W1749586744 title "[5] Catabolism ofl-Ascorbate in animal tissues" @default.
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- W1749586744 doi "https://doi.org/10.1016/0076-6879(71)18273-0" @default.
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