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- W1750147358 abstract "We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce DNA, a kanamycin resistance cassette was inserted into a cloned fragment of phage DNA, and the resulting construct was transformed into B. burgdorferi CA-11.2A cells. The kan cassette recombined into a resident cp32 and was stably maintained. The cp32 containing the kan cassette was packaged by phiBB-1 released from this B. burgdorferi strain. phiBB-1 has been used to transduce this antibiotic resistance marker into naive CA-11.2A cells, as well as two other strains of B. burgdorferi. This is the first direct evidence of a mechanism for lateral gene transfer in B. burgdorferi." @default.
- W1750147358 created "2016-06-24" @default.
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- W1750147358 date "2001-08-15" @default.
- W1750147358 modified "2023-10-16" @default.
- W1750147358 title "Transduction by φBB-1, a Bacteriophage of <i>Borrelia burgdorferi</i>" @default.
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- W1750147358 doi "https://doi.org/10.1128/jb.183.16.4771-4778.2001" @default.
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