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- W175020921 abstract "Publisher Summary Cytidine-5′-diphosphoglucose (CDP-glucose) occurs in a number of microorganisms, where it serves as a direct or indirect precursor of polysaccharides. CDP-glucose is quantitatively converted to CDP-4-keto-6-deoxyglucose by the enzyme CDP-glucose dehydratase from Salmonella typhimurium. The catalytic amounts of exogenous NAD are required for this reaction. The method has its application in biochemistry and microbiology. The principle involved in the method is that the treatment of the keto sugar formed with alkali gives an enediol with an extinction maximum at 318 nm. The increase in extinction at 318 nm is a measure of the amount of CDP-glucose present. This method was first described by Matsuhashi et al. This chapter describes the optimum conditions for the measurement of CDP-glucose. The enzymatic reaction should be carried out between pH 8.0 and 9.0 to obtain a maximum rate of reaction. The enzyme is inhibited by high concentrations of phosphate and is sensitive to heavy metals; therefore, the measurements are carried out in tris buffer with the addition of ethylenediaminetetra-acetic acid (EDTA)." @default.
- W175020921 created "2016-06-24" @default.
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- W175020921 date "1974-01-01" @default.
- W175020921 modified "2023-09-26" @default.
- W175020921 title "Cytidine-5′-diphosphoglucose" @default.
- W175020921 cites W1500729543 @default.
- W175020921 doi "https://doi.org/10.1016/b978-0-12-091304-6.50083-5" @default.
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