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• W175072153 abstract "Transcriptome analysis by next-generation sequencing (RNA-seq) allows investigation of a transcriptome at unsurpassed resolution. One major benefit is that RNA-seq is independent of a priori knowledge on the sequence under investigation, thereby also allowing analysis of poorly characterized Plasmodium species. Here we provide a detailed protocol for RNA isolation and fragmentation, ribosomal RNA depletion, and cDNA synthesis that enables the preparation of a sequencing library from 1 to 2 μg of total RNA. Although we focus our discussion on the quantitative measurement of gene expression, this protocol is suited for many applications of RNA-seq and allows analysis of most RNA species." @default.
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• W175072153 date "2012-01-01" @default.
• W175072153 modified "2023-10-16" @default.
• W175072153 title "Transcriptome Analysis Using RNA-Seq" @default.
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• W175072153 doi "https://doi.org/10.1007/978-1-62703-026-7_15" @default.
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