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• W1750951357 abstract "We aimed to characterize the genetic diversity of drug-resistant Mycobacterium tuberculosis (MTb) clinical isolates and investigate the molecular epidemiology of multidrug-resistant (MDR) tuberculosis from Minas Gerais State, Brazil.One hundred and four MTb clinical isolates were assessed by IS6110-RFLP, 24-locus mycobacterial interspersed repetitive units variable-number tandem repeats (MIRU-VNTR), TB-SPRINT (simultaneous spoligotyping and rifampicin-isoniazid drug-resistance mutation analysis) and 3R-SNP-typing (analysis of single-nucleotide polymorphisms in the genes involved in replication, recombination and repair functions).Fifty-seven different IS6110-RFLP patterns were found, among which 50 had unique patterns and 17 were grouped into seven clusters. The discriminatory index (Hunter and Gaston, HGDI) for RFLP was 0.9937. Ninety-nine different MIRU-VNTR patterns were found, 95 of which had unique patterns and nine isolates were grouped into four clusters. The major allelic diversity index in the MIRU-VNTR loci ranged from 0.6568 to 0.7789. The global HGDI for MIRU-VNTR was 0.9991. Thirty-two different spoligotyping profiles were found: 16 unique patterns (n = 16) and 16 clustered profiles (n = 88). The HGDI for spoligotyping was 0.9009. The spoligotyped clinical isolates were phylogenetically classified into Latin-American Mediterranean (66.34 %), T (14.42 %), Haarlem (5.76 %), X (1.92 %), S (1.92 %) and U (unknown profile; 8.65 %). Among the U isolates, 77.8 % were classified further by 3R-SNP-typing as 44.5 % Haarlem and 33.3 % LAM, while the 22.2 % remaining were not classified. Among the 104 clinical isolates, 86 were identified by TB-SPRINT as MDR, 12 were resistant to rifampicin only, one was resistant to isoniazid only, three were susceptible to both drugs, and two were not successfully amplified by PCR. A total of 42, 28 and eight isolates had mutations in rpoB positions 531, 526 and 516, respectively. Correlating the cluster analysis with the patient data did not suggest recent transmission of MDR-TB.Although our results do not suggest strong transmission of MDR-TB in Minas Gerais (using a classical 100 % MDR-TB identical isolates cluster definition), use of a smoother cluster definition (>85 % similarity) does not allow us to fully eliminate this possibility; hence, around 20-30 % of the isolates we analyzed might be MDR-TB transmission cases." @default.
• W1750951357 created "2016-06-24" @default.
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• W1750951357 date "2015-08-01" @default.
• W1750951357 modified "2023-10-08" @default.
• W1750951357 title "Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil" @default.
• W1750951357 cites W125544664 @default.
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• W1750951357 cites W1540745773 @default.
• W1750951357 cites W1566877505 @default.
• W1750951357 cites W1837184534 @default.
• W1750951357 cites W1844831948 @default.
• W1750951357 cites W1915114500 @default.
• W1750951357 cites W1964501807 @default.
• W1750951357 cites W1966340693 @default.
• W1750951357 cites W1974878042 @default.
• W1750951357 cites W1980446495 @default.
• W1750951357 cites W1982783224 @default.
• W1750951357 cites W1989597041 @default.
• W1750951357 cites W1989652820 @default.
• W1750951357 cites W2001140762 @default.
• W1750951357 cites W2003025878 @default.
• W1750951357 cites W2007707812 @default.
• W1750951357 cites W2012473911 @default.
• W1750951357 cites W2026415706 @default.
• W1750951357 cites W2031599370 @default.
• W1750951357 cites W2039644527 @default.
• W1750951357 cites W2046763412 @default.
• W1750951357 cites W2048894441 @default.
• W1750951357 cites W2063477045 @default.
• W1750951357 cites W2068148879 @default.
• W1750951357 cites W2069468367 @default.
• W1750951357 cites W2071490229 @default.
• W1750951357 cites W2072187332 @default.
• W1750951357 cites W2075429769 @default.
• W1750951357 cites W2078397175 @default.
• W1750951357 cites W2090264937 @default.
• W1750951357 cites W2098618245 @default.
• W1750951357 cites W2100369468 @default.
• W1750951357 cites W2100887397 @default.
• W1750951357 cites W2102914435 @default.
• W1750951357 cites W2105255620 @default.
• W1750951357 cites W2106676963 @default.
• W1750951357 cites W2109910592 @default.
• W1750951357 cites W2109921486 @default.
• W1750951357 cites W2111582999 @default.
• W1750951357 cites W2112101122 @default.
• W1750951357 cites W2116821066 @default.
• W1750951357 cites W2117286584 @default.
• W1750951357 cites W2119119368 @default.
• W1750951357 cites W2119989163 @default.
• W1750951357 cites W2120937842 @default.
• W1750951357 cites W2126568957 @default.
• W1750951357 cites W2131400724 @default.
• W1750951357 cites W2131732398 @default.
• W1750951357 cites W2132026894 @default.
• W1750951357 cites W2132384500 @default.
• W1750951357 cites W2132397638 @default.
• W1750951357 cites W2132545979 @default.
• W1750951357 cites W2133488722 @default.
• W1750951357 cites W2134558351 @default.
• W1750951357 cites W2138474951 @default.
• W1750951357 cites W2139803213 @default.
• W1750951357 cites W2142392294 @default.
• W1750951357 cites W2143367104 @default.
• W1750951357 cites W2143574749 @default.
• W1750951357 cites W2147571700 @default.
• W1750951357 cites W2148453601 @default.
• W1750951357 cites W2149324946 @default.
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• W1750951357 cites W2156887574 @default.
• W1750951357 cites W2160348646 @default.
• W1750951357 cites W2165822919 @default.
• W1750951357 cites W2166893325 @default.
• W1750951357 cites W2318136092 @default.
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• W1750951357 doi "https://doi.org/10.1186/s12879-015-1057-y" @default.
• W1750951357 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4521345" @default.
• W1750951357 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26231661" @default.
• W1750951357 hasPublicationYear "2015" @default.
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