Matches in SemOpenAlex for { <https://semopenalex.org/work/W1751150147> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W1751150147 endingPage "024" @default.
- W1751150147 startingPage "015" @default.
- W1751150147 abstract "Optical spectroscopic techniques can be used to measure Ca2+-binding constants when the Ca2+-bound and free forms of the protein display a difference in, for example, the UV absorbance, CD or fluorescence spectrum, or fluorescence polarization. One may then start with the Ca2+-free form, titrate in Ca2+ stepwise, measure a spectrum or intensity at each step, and obtain the binding constants from computer fitting to the data. The best accuracy is achieved when the protein concentration is roughly the same as the dissociation constant (the inverse of the binding constant) such that there are significant populations of both bound and free forms at several titration points. This limits the useful range of such direct measurements to binding constants below 106 M-1 (KD> 1 μM), because of the practical difficulty of making buffers with less than 0.5-1 μM free Ca2+. For Ca2+-binding proteins with affinities of 106 M-1 and up, one has to rely on indirect measurements. One popular such approach uses around 1 mM ethylenediaminetetracetic acid (EDTA) or ethylene glycol-bis N,N,N′,N′-tetraacetic acid (EGTA), and a much smaller amount of protein so that the free-Ca2+concentration is essentially controlled by the Ca2+-buffering capacity of EDTA or EGTA. A potential risk with such approaches is binding of EDTA or EGTA to the protein with consequences for its Ca2+affinity. Another type of indirect approach outlined in this chapter involves the use of a chelator whose absorbance or fluorescence is Ca2+dependent (1-3)." @default.
- W1751150147 created "2016-06-24" @default.
- W1751150147 creator A5070625264 @default.
- W1751150147 date "2003-11-14" @default.
- W1751150147 modified "2023-09-23" @default.
- W1751150147 title "Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators" @default.
- W1751150147 cites W1606920154 @default.
- W1751150147 cites W1974761584 @default.
- W1751150147 cites W1984671539 @default.
- W1751150147 cites W2007838869 @default.
- W1751150147 cites W2008459956 @default.
- W1751150147 cites W2058449890 @default.
- W1751150147 cites W2059112746 @default.
- W1751150147 doi "https://doi.org/10.1385/1-59259-184-1:015" @default.
- W1751150147 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11859758" @default.
- W1751150147 hasPublicationYear "2003" @default.
- W1751150147 type Work @default.
- W1751150147 sameAs 1751150147 @default.
- W1751150147 citedByCount "18" @default.
- W1751150147 countsByYear W17511501472016 @default.
- W1751150147 countsByYear W17511501472018 @default.
- W1751150147 countsByYear W17511501472020 @default.
- W1751150147 countsByYear W17511501472021 @default.
- W1751150147 crossrefType "book-chapter" @default.
- W1751150147 hasAuthorship W1751150147A5070625264 @default.
- W1751150147 hasConcept C107824862 @default.
- W1751150147 hasConcept C113196181 @default.
- W1751150147 hasConcept C121332964 @default.
- W1751150147 hasConcept C170493617 @default.
- W1751150147 hasConcept C178790620 @default.
- W1751150147 hasConcept C179104552 @default.
- W1751150147 hasConcept C184866935 @default.
- W1751150147 hasConcept C185592680 @default.
- W1751150147 hasConcept C197404232 @default.
- W1751150147 hasConcept C2777658833 @default.
- W1751150147 hasConcept C2780283098 @default.
- W1751150147 hasConcept C32786932 @default.
- W1751150147 hasConcept C43617362 @default.
- W1751150147 hasConcept C519063684 @default.
- W1751150147 hasConcept C55493867 @default.
- W1751150147 hasConcept C62520636 @default.
- W1751150147 hasConcept C71240020 @default.
- W1751150147 hasConcept C74998103 @default.
- W1751150147 hasConcept C8010536 @default.
- W1751150147 hasConcept C91881484 @default.
- W1751150147 hasConcept C98015330 @default.
- W1751150147 hasConceptScore W1751150147C107824862 @default.
- W1751150147 hasConceptScore W1751150147C113196181 @default.
- W1751150147 hasConceptScore W1751150147C121332964 @default.
- W1751150147 hasConceptScore W1751150147C170493617 @default.
- W1751150147 hasConceptScore W1751150147C178790620 @default.
- W1751150147 hasConceptScore W1751150147C179104552 @default.
- W1751150147 hasConceptScore W1751150147C184866935 @default.
- W1751150147 hasConceptScore W1751150147C185592680 @default.
- W1751150147 hasConceptScore W1751150147C197404232 @default.
- W1751150147 hasConceptScore W1751150147C2777658833 @default.
- W1751150147 hasConceptScore W1751150147C2780283098 @default.
- W1751150147 hasConceptScore W1751150147C32786932 @default.
- W1751150147 hasConceptScore W1751150147C43617362 @default.
- W1751150147 hasConceptScore W1751150147C519063684 @default.
- W1751150147 hasConceptScore W1751150147C55493867 @default.
- W1751150147 hasConceptScore W1751150147C62520636 @default.
- W1751150147 hasConceptScore W1751150147C71240020 @default.
- W1751150147 hasConceptScore W1751150147C74998103 @default.
- W1751150147 hasConceptScore W1751150147C8010536 @default.
- W1751150147 hasConceptScore W1751150147C91881484 @default.
- W1751150147 hasConceptScore W1751150147C98015330 @default.
- W1751150147 hasLocation W17511501471 @default.
- W1751150147 hasLocation W17511501472 @default.
- W1751150147 hasOpenAccess W1751150147 @default.
- W1751150147 hasPrimaryLocation W17511501471 @default.
- W1751150147 hasRelatedWork W1751150147 @default.
- W1751150147 hasRelatedWork W2011727025 @default.
- W1751150147 hasRelatedWork W2019409288 @default.
- W1751150147 hasRelatedWork W2024683779 @default.
- W1751150147 hasRelatedWork W2034017121 @default.
- W1751150147 hasRelatedWork W2056142580 @default.
- W1751150147 hasRelatedWork W2065442827 @default.
- W1751150147 hasRelatedWork W2117024383 @default.
- W1751150147 hasRelatedWork W304683614 @default.
- W1751150147 hasRelatedWork W2182096203 @default.
- W1751150147 isParatext "false" @default.
- W1751150147 isRetracted "false" @default.
- W1751150147 magId "1751150147" @default.
- W1751150147 workType "book-chapter" @default.