FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1751196650> ?p ?o ?g. }

• W1751196650 abstract "Human tissue Factor Pathway Inhibitor-2 (TFPI-2) is a potent inhibitor of plasmin, which activates metalloproteinases involved in extracellular matrix degradation. Its secretion in the extracellular matrix makes TFPI-2 a potential inhibitor of tumor cell invasion. However, no studies have yet evaluated the wound-healing activities of human Tenon's capsule fibroblasts (hTCFs). The aim of the study is to elucidate the effect of TFPI-2 overexpression on hTCF proliferation and migration, to determine whether TFPI-2 may act as an antiscarring agent in vivo after glaucoma filtration surgery.Plasmid vector pBos-Cite-neo/TFPI-2 was transfected into hTCFs with Lipofectamine 2000. After selection by G418, transfected, non-transfected, and mock-transfected cells were screened for TFPI-2 mRNA and protein by reverse transcription-PCR and western blot analysis respectively. Cell proliferation and viability were determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry. Cell migration was studied on restrained collagen gels and with a scratch-wound assay.TFPI-2 expression of mRNA and protein was confirmed in transfected cells. The transfected, non-transfected, and mock-transfected cells showed no significant difference in cell proliferation and apoptosis, with TFPI-2 found not to be cytotoxic in hTCFs. Overexpression of TFPI-2 significantly suppressed cell migration three- to four-fold on collagen gel for 2 weeks and in the scratch-wound assay for 2 d (39.27+/-2.40% versus 16.43+/-1.10% at 1 d, and 79.0+/-3.04% versus 30.13+/-2.1% at 2 d).TFPI-2 expression may strongly inhibit the migration ability of hTCFs in vitro, making it a promising candidate for novel therapies to minimize scar development after glaucoma drainage surgery." @default.
• W1751196650 created "2016-06-24" @default.
• W1751196650 creator A5007212758 @default.
• W1751196650 creator A5025205138 @default.
• W1751196650 date "2009-11-12" @default.
• W1751196650 modified "2023-09-23" @default.
• W1751196650 title "Human tissue factor pathway inhibitor-2 suppresses the wound-healing activities of human Tenon's capsule fibroblasts in vitro." @default.
• W1751196650 cites W1540750011 @default.
• W1751196650 cites W1964357828 @default.
• W1751196650 cites W1975618693 @default.
• W1751196650 cites W2000510976 @default.
• W1751196650 cites W2010164749 @default.
• W1751196650 cites W2026740600 @default.
• W1751196650 cites W2029727986 @default.
• W1751196650 cites W2065800006 @default.
• W1751196650 cites W2075551181 @default.
• W1751196650 cites W2083431276 @default.
• W1751196650 cites W2089357765 @default.
• W1751196650 cites W2116817570 @default.
• W1751196650 cites W2129377727 @default.
• W1751196650 cites W2246769555 @default.
• W1751196650 cites W51703526 @default.
• W1751196650 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2779065" @default.
• W1751196650 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19936309" @default.
• W1751196650 hasPublicationYear "2009" @default.
• W1751196650 type Work @default.
• W1751196650 sameAs 1751196650 @default.
• W1751196650 citedByCount "3" @default.
• W1751196650 countsByYear W17511966502012 @default.
• W1751196650 countsByYear W17511966502023 @default.
• W1751196650 crossrefType "journal-article" @default.
• W1751196650 hasAuthorship W1751196650A5007212758 @default.
• W1751196650 hasAuthorship W1751196650A5025205138 @default.
• W1751196650 hasConcept C109523444 @default.
• W1751196650 hasConcept C126322002 @default.
• W1751196650 hasConcept C137738243 @default.
• W1751196650 hasConcept C150903083 @default.
• W1751196650 hasConcept C181199279 @default.
• W1751196650 hasConcept C185592680 @default.
• W1751196650 hasConcept C186738567 @default.
• W1751196650 hasConcept C189165786 @default.
• W1751196650 hasConcept C202751555 @default.
• W1751196650 hasConcept C203014093 @default.
• W1751196650 hasConcept C207001950 @default.
• W1751196650 hasConcept C2775965246 @default.
• W1751196650 hasConcept C2777151630 @default.
• W1751196650 hasConcept C2778382381 @default.
• W1751196650 hasConcept C2780269544 @default.
• W1751196650 hasConcept C2781071845 @default.
• W1751196650 hasConcept C49039625 @default.
• W1751196650 hasConcept C502942594 @default.
• W1751196650 hasConcept C55493867 @default.
• W1751196650 hasConcept C71924100 @default.
• W1751196650 hasConcept C86803240 @default.
• W1751196650 hasConcept C95444343 @default.
• W1751196650 hasConcept C98274493 @default.
• W1751196650 hasConceptScore W1751196650C109523444 @default.
• W1751196650 hasConceptScore W1751196650C126322002 @default.
• W1751196650 hasConceptScore W1751196650C137738243 @default.
• W1751196650 hasConceptScore W1751196650C150903083 @default.
• W1751196650 hasConceptScore W1751196650C181199279 @default.
• W1751196650 hasConceptScore W1751196650C185592680 @default.
• W1751196650 hasConceptScore W1751196650C186738567 @default.
• W1751196650 hasConceptScore W1751196650C189165786 @default.
• W1751196650 hasConceptScore W1751196650C202751555 @default.
• W1751196650 hasConceptScore W1751196650C203014093 @default.
• W1751196650 hasConceptScore W1751196650C207001950 @default.
• W1751196650 hasConceptScore W1751196650C2775965246 @default.
• W1751196650 hasConceptScore W1751196650C2777151630 @default.
• W1751196650 hasConceptScore W1751196650C2778382381 @default.
• W1751196650 hasConceptScore W1751196650C2780269544 @default.
• W1751196650 hasConceptScore W1751196650C2781071845 @default.
• W1751196650 hasConceptScore W1751196650C49039625 @default.
• W1751196650 hasConceptScore W1751196650C502942594 @default.
• W1751196650 hasConceptScore W1751196650C55493867 @default.
• W1751196650 hasConceptScore W1751196650C71924100 @default.
• W1751196650 hasConceptScore W1751196650C86803240 @default.
• W1751196650 hasConceptScore W1751196650C95444343 @default.
• W1751196650 hasConceptScore W1751196650C98274493 @default.
• W1751196650 hasLocation W17511966501 @default.
• W1751196650 hasOpenAccess W1751196650 @default.
• W1751196650 hasPrimaryLocation W17511966501 @default.
• W1751196650 hasRelatedWork W1152007223 @default.
• W1751196650 hasRelatedWork W1770301119 @default.
• W1751196650 hasRelatedWork W1934340033 @default.
• W1751196650 hasRelatedWork W1973421418 @default.
• W1751196650 hasRelatedWork W1989305669 @default.
• W1751196650 hasRelatedWork W1992770651 @default.
• W1751196650 hasRelatedWork W2020809515 @default.
• W1751196650 hasRelatedWork W2025698806 @default.
• W1751196650 hasRelatedWork W2077442923 @default.
• W1751196650 hasRelatedWork W2092250612 @default.
• W1751196650 hasRelatedWork W2151209025 @default.
• W1751196650 hasRelatedWork W2155768339 @default.
• W1751196650 hasRelatedWork W2236886743 @default.
• W1751196650 hasRelatedWork W2313956146 @default.
• W1751196650 hasRelatedWork W2529035533 @default.
• W1751196650 hasRelatedWork W2884474077 @default.
• W1751196650 hasRelatedWork W2960541941 @default.
• W1751196650 hasRelatedWork W2990548152 @default.

• next