Matches in SemOpenAlex for { <https://semopenalex.org/work/W175249920> ?p ?o ?g. }
- W175249920 endingPage "503" @default.
- W175249920 startingPage "498" @default.
- W175249920 abstract "In the field of gene therapy, viral vectors as delivery tools have a number of disadvantages for medical application. This study aimed to explore a novel nonviral vector as a vehicle for gene therapy.Transvector-rpE-MPP and EGFP (enhanced green fluorescent protein) were used as the gene transfer carrier and the reporter gene, respectively. Polyplexes which integrate transvector-rpE-MPP, the object gene, and EGFP were formed. The optimal charge ratio, stability, and transduction capacity of the polyplexes in mouse hepatocytes in vitro and in mouse liver in vivo were investigated. The polyplexes of transvector-rpE-MPP and pcDNA(3)-EGFP, with charge ratios of 0, 0.25, 0.5, 0.75, 1 and 1.5 were compared to determine the optimal charge ratio.Polyplexes with charge ratios of 1:1 were most stable; pcDNA(3)-EGFP in these complexes resisted digestion by DNase I and blood plasma. On the other hand, pcDNA(3)-EGFP alone was digested. Fluorescence analysis indicated that transvector-rpE-MPP successfully delivered the reporter gene EGFP into hepatocytes and that EGFP expression was detected in hepatocyte cultures and in liver tissue.These results have laid a foundation for further study of a novel nonviral gene delivery system." @default.
- W175249920 created "2016-06-24" @default.
- W175249920 creator A5008857498 @default.
- W175249920 creator A5023858361 @default.
- W175249920 creator A5026779660 @default.
- W175249920 creator A5039717240 @default.
- W175249920 creator A5065075495 @default.
- W175249920 creator A5066094492 @default.
- W175249920 date "2009-10-01" @default.
- W175249920 modified "2023-09-25" @default.
- W175249920 title "Hepatocyte gene transfer mediated by stable polyplexes based on MPP-containing DNA complexes." @default.
- W175249920 cites W126651838 @default.
- W175249920 cites W1520821663 @default.
- W175249920 cites W1525454261 @default.
- W175249920 cites W1534317533 @default.
- W175249920 cites W1576238436 @default.
- W175249920 cites W1579604458 @default.
- W175249920 cites W1584683177 @default.
- W175249920 cites W1826836006 @default.
- W175249920 cites W1970812755 @default.
- W175249920 cites W1971290197 @default.
- W175249920 cites W1975541922 @default.
- W175249920 cites W1988594716 @default.
- W175249920 cites W1992156570 @default.
- W175249920 cites W2006724257 @default.
- W175249920 cites W2009508594 @default.
- W175249920 cites W2010655741 @default.
- W175249920 cites W2012694727 @default.
- W175249920 cites W2016731705 @default.
- W175249920 cites W2021783595 @default.
- W175249920 cites W2023934367 @default.
- W175249920 cites W2024362853 @default.
- W175249920 cites W2024706828 @default.
- W175249920 cites W2025976409 @default.
- W175249920 cites W2034642422 @default.
- W175249920 cites W2056842925 @default.
- W175249920 cites W2058408260 @default.
- W175249920 cites W2074877984 @default.
- W175249920 cites W2079335891 @default.
- W175249920 cites W2079740287 @default.
- W175249920 cites W2085603447 @default.
- W175249920 cites W2090559773 @default.
- W175249920 cites W2104823981 @default.
- W175249920 cites W2120567214 @default.
- W175249920 cites W2121944814 @default.
- W175249920 cites W2129859001 @default.
- W175249920 cites W2134000542 @default.
- W175249920 cites W2144178772 @default.
- W175249920 cites W2160902183 @default.
- W175249920 cites W2327537299 @default.
- W175249920 cites W2357072072 @default.
- W175249920 cites W2359971417 @default.
- W175249920 cites W2395500600 @default.
- W175249920 cites W97395653 @default.
- W175249920 cites W228278199 @default.
- W175249920 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19822493" @default.
- W175249920 hasPublicationYear "2009" @default.
- W175249920 type Work @default.
- W175249920 sameAs 175249920 @default.
- W175249920 citedByCount "1" @default.
- W175249920 crossrefType "journal-article" @default.
- W175249920 hasAuthorship W175249920A5008857498 @default.
- W175249920 hasAuthorship W175249920A5023858361 @default.
- W175249920 hasAuthorship W175249920A5026779660 @default.
- W175249920 hasAuthorship W175249920A5039717240 @default.
- W175249920 hasAuthorship W175249920A5065075495 @default.
- W175249920 hasAuthorship W175249920A5066094492 @default.
- W175249920 hasConcept C104317684 @default.
- W175249920 hasConcept C111599444 @default.
- W175249920 hasConcept C12554922 @default.
- W175249920 hasConcept C135983454 @default.
- W175249920 hasConcept C142613039 @default.
- W175249920 hasConcept C150194340 @default.
- W175249920 hasConcept C15152581 @default.
- W175249920 hasConcept C153911025 @default.
- W175249920 hasConcept C161733203 @default.
- W175249920 hasConcept C202751555 @default.
- W175249920 hasConcept C207001950 @default.
- W175249920 hasConcept C2776200302 @default.
- W175249920 hasConcept C3018928802 @default.
- W175249920 hasConcept C32470452 @default.
- W175249920 hasConcept C40767141 @default.
- W175249920 hasConcept C54009773 @default.
- W175249920 hasConcept C54355233 @default.
- W175249920 hasConcept C55493867 @default.
- W175249920 hasConcept C86803240 @default.
- W175249920 hasConcept C95444343 @default.
- W175249920 hasConceptScore W175249920C104317684 @default.
- W175249920 hasConceptScore W175249920C111599444 @default.
- W175249920 hasConceptScore W175249920C12554922 @default.
- W175249920 hasConceptScore W175249920C135983454 @default.
- W175249920 hasConceptScore W175249920C142613039 @default.
- W175249920 hasConceptScore W175249920C150194340 @default.
- W175249920 hasConceptScore W175249920C15152581 @default.
- W175249920 hasConceptScore W175249920C153911025 @default.
- W175249920 hasConceptScore W175249920C161733203 @default.
- W175249920 hasConceptScore W175249920C202751555 @default.
- W175249920 hasConceptScore W175249920C207001950 @default.