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• W1752617402 abstract "Rare conjugational progeny formed by crossing each of five Hfr strains with a recA-F- strain have been characterized. Selection was made for a proximal Hfr marker, taking strict precautions to prevent transfer of recA+ to the zygotes. Most of the progeny were found to be F' strains containing deletion mutant plasmids. With two exceptions, these mutant plasmids have lost all of the tra genes, which are required to confer conjugational donor ability upon a host. In addition, all but the exceptional mutant plasmids were found to be very poorly transmissible from transient heterozygotes which also contain a wild-type F' plasmid. The poor transmissibility is a cis-dominant transfer-defective phenotype which may result from deletion of all or part of the origin of transfer replication (ori), or of a gene determining a cis-acting protein. The two exceptional mutant plasmids may carry short deletions of some of the tra genes or polar tra mutations. The remaining progeny were nonmutant F' strains and F- strains. The frequency with which the F- strains were recovered permits us to estimate that the maximum amount of recombination possible in a recA56 zygote is 10(-6) that of a recA+ zygote." @default.
• W1752617402 created "2016-06-24" @default.
• W1752617402 creator A5000299667 @default.
• W1752617402 creator A5085415256 @default.
• W1752617402 date "1976-01-01" @default.
• W1752617402 modified "2023-10-14" @default.
• W1752617402 title "cis-Dominant, transfer-deficient mutants of the Escherichia coli K-12 F sex factor" @default.
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• W1752617402 doi "https://doi.org/10.1128/jb.125.1.233-247.1976" @default.
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