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- W1755018410 abstract "Abstract In the current study, we have investigated the ability of substance P (SP) to protect 3‐day‐old (P3) rat spiral ganglion neurons (SGNs) from trophic factor deprivation (TFD)‐induced cell death. The presence of SP high affinity neurokinin‐1 receptor (NK1) transcripts was detected in the spiral ganglion and the NK1 protein localized to SGNs both ex vivo and in vitro . Treatment with SP increased cytoplasmic Ca 2+ in SGNs, further arguing for the presence of functional NK1 on these neurons. Both SP and the agonist [Sar 9 ,Met(O 2 ) 11 ]‐SP significantly decreased SGN cell death induced by TFD, with no effect on neurite outgrowth. The survival promoting effect of SP was blocked by the NK1 antagonist, WIN51708. Both pan‐caspase inhibitor BOC‐D‐FMK and SP treatments markedly reduced activation of caspases and DNA fragmentation in trophic factor deprived‐neurons. The neuroprotective action of SP was antagonised by specific inhibitors of second messengers, including 1.2‐bis‐(O‐aminophenoxy)‐ethane‐ N , N , N ′, N ′‐tetraacetic acid (BAPTA‐AM) to chelate cytosolic Ca 2+ , the protein kinase C (PKC) inhibitors bisindolylmaleimide I, Gö6976 and LY333531 and the MAPK/ERK inhibitor U0126. In contrast, nifedipine, a specific inhibitor of l ‐type Ca 2+ channel, and LY294002, a phosphatidylinositol‐3‐OH kinase (PI3K) inhibitor, had no effect on SP trophic support of SGNs. Moreover, activation of endogenous PKC by 4β‐phorbol 12‐myristate 13‐acetate (PMA) also reduced the loss of trophic factor‐deprived SGNs. Thus, NK1 expressed by SGNs transmit a survival‐promoting regulatory signal during TFD‐induced SGN cell death via pathways involving PKC activation, Ca 2+ signalling and MAPK/ERK activation, which can be accounted for by an inhibition of caspase activation." @default.
- W1755018410 created "2016-06-24" @default.
- W1755018410 creator A5026359213 @default.
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- W1755018410 creator A5055029223 @default.
- W1755018410 creator A5055112042 @default.
- W1755018410 creator A5058303994 @default.
- W1755018410 date "2003-09-17" @default.
- W1755018410 modified "2023-10-16" @default.
- W1755018410 title "Substance P protects spiral ganglion neurons from apoptosis via PKC-Ca2+-MAPK/ERK pathways" @default.
- W1755018410 cites W1515601041 @default.
- W1755018410 cites W1518732132 @default.
- W1755018410 cites W152459441 @default.
- W1755018410 cites W1570910223 @default.
- W1755018410 cites W1573000660 @default.
- W1755018410 cites W1576671418 @default.
- W1755018410 cites W1596652845 @default.
- W1755018410 cites W1641731911 @default.
- W1755018410 cites W1739139252 @default.
- W1755018410 cites W1755902553 @default.
- W1755018410 cites W1809136836 @default.
- W1755018410 cites W1822066871 @default.
- W1755018410 cites W1860985526 @default.
- W1755018410 cites W1886438078 @default.
- W1755018410 cites W1933897045 @default.
- W1755018410 cites W1946776760 @default.
- W1755018410 cites W1963831745 @default.
- W1755018410 cites W1975646316 @default.
- W1755018410 cites W1975823509 @default.
- W1755018410 cites W1975915718 @default.
- W1755018410 cites W1975995627 @default.
- W1755018410 cites W1978004673 @default.
- W1755018410 cites W1979032136 @default.
- W1755018410 cites W1980166477 @default.
- W1755018410 cites W1982584664 @default.
- W1755018410 cites W1992965538 @default.
- W1755018410 cites W1994497941 @default.
- W1755018410 cites W1994816216 @default.
- W1755018410 cites W1997926000 @default.
- W1755018410 cites W1998225973 @default.
- W1755018410 cites W1998936456 @default.
- W1755018410 cites W1999514420 @default.
- W1755018410 cites W1999788892 @default.
- W1755018410 cites W2006052107 @default.
- W1755018410 cites W2007448080 @default.
- W1755018410 cites W2008032373 @default.
- W1755018410 cites W2009071202 @default.
- W1755018410 cites W2010996478 @default.
- W1755018410 cites W2012016665 @default.
- W1755018410 cites W2012746210 @default.
- W1755018410 cites W2013558874 @default.
- W1755018410 cites W2014663128 @default.
- W1755018410 cites W2014705617 @default.
- W1755018410 cites W2017903916 @default.
- W1755018410 cites W2018071393 @default.
- W1755018410 cites W2019128864 @default.
- W1755018410 cites W2021516892 @default.
- W1755018410 cites W2024321889 @default.
- W1755018410 cites W2025227173 @default.
- W1755018410 cites W2035266661 @default.
- W1755018410 cites W2040954045 @default.
- W1755018410 cites W2048145892 @default.
- W1755018410 cites W2048302926 @default.
- W1755018410 cites W2048914428 @default.
- W1755018410 cites W2051106291 @default.
- W1755018410 cites W2051279593 @default.
- W1755018410 cites W2051942658 @default.
- W1755018410 cites W2054315641 @default.
- W1755018410 cites W2054484850 @default.
- W1755018410 cites W2055730141 @default.
- W1755018410 cites W2057250008 @default.
- W1755018410 cites W2059214757 @default.
- W1755018410 cites W2061603242 @default.
- W1755018410 cites W2064295865 @default.
- W1755018410 cites W2066489411 @default.
- W1755018410 cites W2072300509 @default.
- W1755018410 cites W2073508559 @default.
- W1755018410 cites W2073626626 @default.
- W1755018410 cites W2077631665 @default.
- W1755018410 cites W2081422759 @default.
- W1755018410 cites W2081846788 @default.
- W1755018410 cites W2081991873 @default.
- W1755018410 cites W2089002950 @default.
- W1755018410 cites W2092014913 @default.
- W1755018410 cites W2095063454 @default.
- W1755018410 cites W2097331211 @default.
- W1755018410 cites W2104969843 @default.
- W1755018410 cites W2109483196 @default.
- W1755018410 cites W2110196938 @default.
- W1755018410 cites W2114307187 @default.
- W1755018410 cites W2125797096 @default.
- W1755018410 cites W2130895186 @default.
- W1755018410 cites W2142766154 @default.
- W1755018410 cites W2149287700 @default.
- W1755018410 cites W2153990155 @default.
- W1755018410 cites W2154989101 @default.