FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1759014671> ?p ?o ?g. }

• W1759014671 endingPage "3579" @default.
• W1759014671 startingPage "3570" @default.
• W1759014671 abstract "Primary cultures of hepatocytes isolated by collagenase perfusion of adult rats were transformed by infection with adenovirus type 5 or transfection with adenovirus DNA. Total virion DNA or recombinant plasmid DNA containing the adenovirus E1A and E1B genes transformed hepatocytes at comparable frequencies. No foci of replicating hepatocytes were detected after transfection with a plasmid containing the E1A gene alone. The frequency of transformation by the adenovirus E1A and E1B genes was dependent on the composition of the culture medium. Transformation occurred at a low frequency when the transfected hepatocytes were maintained in a chemically defined medium (CDM), but the frequency was enhanced 8- to 10-fold when the cells were maintained in (i) serum-supplemented medium or (ii) CDM supplemented with epidermal growth factor. Cell lines derived from the adenovirus-transformed colonies of hepatocytes expressed adenovirus E1A and E1B RNAs. When hepatocytes were maintained in CDM supplemented with dimethyl sulfoxide and transfected with plasmids containing the E1A and E1B genes, it was possible to derive cell lines that retained the ability to express several liver-specific genes, including albumin, transferrin, hemopexin, and the third component of complement. The amount of albumin secreted per cell varied from 1 to 5 pg per cell per 24 h, and in one cell line it was below detectable levels by passage 9. Adenovirus-transformed hepatocytes were not tumorigenic when inoculated subcutaneously into neonatal syngeneic rats. We conclude that the adenovirus E1A and E1B genes are capable of transforming adult rat hepatocytes, a differentiated epithelial cell type." @default.
• W1759014671 created "2016-06-24" @default.
• W1759014671 creator A5060961843 @default.
• W1759014671 creator A5074676696 @default.
• W1759014671 date "1987-11-01" @default.
• W1759014671 modified "2023-10-17" @default.
• W1759014671 title "Transformation of differentiated rat hepatocytes with adenovirus and adenovirus DNA" @default.
• W1759014671 cites W138435273 @default.
• W1759014671 cites W1558461849 @default.
• W1759014671 cites W1562468599 @default.
• W1759014671 cites W1563895113 @default.
• W1759014671 cites W1575604311 @default.
• W1759014671 cites W1595281725 @default.
• W1759014671 cites W1649600008 @default.
• W1759014671 cites W1834945569 @default.
• W1759014671 cites W1858263251 @default.
• W1759014671 cites W1869766858 @default.
• W1759014671 cites W1965179957 @default.
• W1759014671 cites W1965372133 @default.
• W1759014671 cites W1966973502 @default.
• W1759014671 cites W1988844504 @default.
• W1759014671 cites W1989009382 @default.
• W1759014671 cites W1990986783 @default.
• W1759014671 cites W1995849358 @default.
• W1759014671 cites W2015560811 @default.
• W1759014671 cites W2015943228 @default.
• W1759014671 cites W2018289835 @default.
• W1759014671 cites W2018575551 @default.
• W1759014671 cites W2019871334 @default.
• W1759014671 cites W2020368139 @default.
• W1759014671 cites W2020411245 @default.
• W1759014671 cites W2021827559 @default.
• W1759014671 cites W2026460545 @default.
• W1759014671 cites W2027104051 @default.
• W1759014671 cites W2029987769 @default.
• W1759014671 cites W2030202421 @default.
• W1759014671 cites W2032258076 @default.
• W1759014671 cites W2032313134 @default.
• W1759014671 cites W2034277598 @default.
• W1759014671 cites W2044296969 @default.
• W1759014671 cites W2050795594 @default.
• W1759014671 cites W2052019438 @default.
• W1759014671 cites W2053366463 @default.
• W1759014671 cites W2059043047 @default.
• W1759014671 cites W2059944708 @default.
• W1759014671 cites W2060333964 @default.
• W1759014671 cites W2063337303 @default.
• W1759014671 cites W2069564134 @default.
• W1759014671 cites W2069589902 @default.
• W1759014671 cites W2069812292 @default.
• W1759014671 cites W2072482644 @default.
• W1759014671 cites W2074971250 @default.
• W1759014671 cites W2083856818 @default.
• W1759014671 cites W2088130891 @default.
• W1759014671 cites W2091933515 @default.
• W1759014671 cites W2091942447 @default.
• W1759014671 cites W2092580169 @default.
• W1759014671 cites W2094307886 @default.
• W1759014671 cites W2100837269 @default.
• W1759014671 cites W2110586785 @default.
• W1759014671 cites W2116630730 @default.
• W1759014671 cites W2116951270 @default.
• W1759014671 cites W2117079180 @default.
• W1759014671 cites W2118989941 @default.
• W1759014671 cites W2138820324 @default.
• W1759014671 cites W2139190596 @default.
• W1759014671 cites W2143817317 @default.
• W1759014671 cites W2159072093 @default.
• W1759014671 cites W2162934736 @default.
• W1759014671 cites W2164302190 @default.
• W1759014671 cites W2240627417 @default.
• W1759014671 cites W2417336611 @default.
• W1759014671 cites W4231167851 @default.
• W1759014671 doi "https://doi.org/10.1128/jvi.61.11.3570-3579.1987" @default.
• W1759014671 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/255957" @default.
• W1759014671 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3669153" @default.
• W1759014671 hasPublicationYear "1987" @default.
• W1759014671 type Work @default.
• W1759014671 sameAs 1759014671 @default.
• W1759014671 citedByCount "17" @default.
• W1759014671 countsByYear W17590146712014 @default.
• W1759014671 countsByYear W17590146712019 @default.
• W1759014671 crossrefType "journal-article" @default.
• W1759014671 hasAuthorship W1759014671A5060961843 @default.
• W1759014671 hasAuthorship W1759014671A5074676696 @default.
• W1759014671 hasBestOaLocation W17590146711 @default.
• W1759014671 hasConcept C104317684 @default.
• W1759014671 hasConcept C111599444 @default.
• W1759014671 hasConcept C145085861 @default.
• W1759014671 hasConcept C153911025 @default.
• W1759014671 hasConcept C159047783 @default.
• W1759014671 hasConcept C204241405 @default.
• W1759014671 hasConcept C22744801 @default.
• W1759014671 hasConcept C2522874641 @default.
• W1759014671 hasConcept C2776954459 @default.
• W1759014671 hasConcept C2777046247 @default.
• W1759014671 hasConcept C2781036101 @default.
• W1759014671 hasConcept C54009773 @default.

• next