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• W176017860 startingPage "55" @default.
• W176017860 abstract "With the discovery of several matrices which enable the ionization of DNA and RNA, matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has become a powerful platform for the study of nucleic acid sequence changes (e.g., mutations, single nucleotide polymorphisms (SNPs), insertion/deletion, alternative splicing, etc.), amount changes (e.g., copy number variation, gene expression, allele expression, etc.), as well as modifications (e.g., methylation of genomic DNA, post transcriptional modification of tRNAs and rRNAs). Two major strategies have been employed to characterize these changes. Primer extension reactions are designed for genotyping of known polymorphic sites and determining the levels of gene or allele expressions. Base-specific cleavage reactions are used for discovery of unknown polymorphisms and characterization of modifications. These two assays usually generate nucleic acid fragments less than 30 bases in length, which is the ideal mass range for MALDI-MS. Here we review the basic concepts of these assays, sample analysis techniques, and their applications published in recent years." @default.
• W176017860 created "2016-06-24" @default.
• W176017860 creator A5015413405 @default.
• W176017860 creator A5045075969 @default.
• W176017860 creator A5061121795 @default.
• W176017860 creator A5086043105 @default.
• W176017860 date "2012-01-01" @default.
• W176017860 modified "2023-10-14" @default.
• W176017860 title "MALDI Mass Spectrometry for Nucleic Acid Analysis" @default.
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