Matches in SemOpenAlex for { <https://semopenalex.org/work/W1761541377> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W1761541377 endingPage "816" @default.
- W1761541377 startingPage "801" @default.
- W1761541377 abstract "Abstract A procedure permitting deletion scanning of potential cis-regulatory sequences within a transgene whose genomic position remains fixed was applied to the study of the upstream sequences of the ecdysteroid-inducible Fat-body-protein-1 (Fbp1) gene. Deficiencies were induced in a Fbp1:Adh fusion transgene by means of a secondary P transposase mutagenesis. Phenotypic and molecular screens were used to select mutant transposons that retained their original genomic location and carried a deletion affecting the Fbp1 sequences but not the Adh reporter gene. Molecular mapping of the deletion breakpoints was achieved by sequence analysis and expression of the reporter gene was quantified by measurement of ADH activity. This procedure was efficient in detecting cis-acting elements, even those with moderate effects on levels of gene expression. For example, we have succeeded in identifying a negative regulatory element. Deletion of this element leads to a 50% increase in the reporter ADH activity. This element binds the transcription factor AEF-1. In addition, we have detected a strong, positively acting element contained within a 32-bp region located immediately upstream of an ecdysone-response element." @default.
- W1761541377 created "2016-06-24" @default.
- W1761541377 creator A5007090639 @default.
- W1761541377 creator A5022741376 @default.
- W1761541377 creator A5064442214 @default.
- W1761541377 creator A5071350957 @default.
- W1761541377 date "1993-11-01" @default.
- W1761541377 modified "2023-10-17" @default.
- W1761541377 title "Deletion scanning of the regulatory sequences of the Fbp1 gene of Drosophila melanogaster using P transposase-induced deficiencies." @default.
- W1761541377 doi "https://doi.org/10.1093/genetics/135.3.801" @default.
- W1761541377 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1205721" @default.
- W1761541377 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8293980" @default.
- W1761541377 hasPublicationYear "1993" @default.
- W1761541377 type Work @default.
- W1761541377 sameAs 1761541377 @default.
- W1761541377 citedByCount "10" @default.
- W1761541377 countsByYear W17615413772021 @default.
- W1761541377 crossrefType "journal-article" @default.
- W1761541377 hasAuthorship W1761541377A5007090639 @default.
- W1761541377 hasAuthorship W1761541377A5022741376 @default.
- W1761541377 hasAuthorship W1761541377A5064442214 @default.
- W1761541377 hasAuthorship W1761541377A5071350957 @default.
- W1761541377 hasBestOaLocation W17615413772 @default.
- W1761541377 hasConcept C102230213 @default.
- W1761541377 hasConcept C104317684 @default.
- W1761541377 hasConcept C143065580 @default.
- W1761541377 hasConcept C150194340 @default.
- W1761541377 hasConcept C153911025 @default.
- W1761541377 hasConcept C160775987 @default.
- W1761541377 hasConcept C161733203 @default.
- W1761541377 hasConcept C16318435 @default.
- W1761541377 hasConcept C172510086 @default.
- W1761541377 hasConcept C21592294 @default.
- W1761541377 hasConcept C2780104201 @default.
- W1761541377 hasConcept C4918238 @default.
- W1761541377 hasConcept C54355233 @default.
- W1761541377 hasConcept C86339819 @default.
- W1761541377 hasConcept C86803240 @default.
- W1761541377 hasConceptScore W1761541377C102230213 @default.
- W1761541377 hasConceptScore W1761541377C104317684 @default.
- W1761541377 hasConceptScore W1761541377C143065580 @default.
- W1761541377 hasConceptScore W1761541377C150194340 @default.
- W1761541377 hasConceptScore W1761541377C153911025 @default.
- W1761541377 hasConceptScore W1761541377C160775987 @default.
- W1761541377 hasConceptScore W1761541377C161733203 @default.
- W1761541377 hasConceptScore W1761541377C16318435 @default.
- W1761541377 hasConceptScore W1761541377C172510086 @default.
- W1761541377 hasConceptScore W1761541377C21592294 @default.
- W1761541377 hasConceptScore W1761541377C2780104201 @default.
- W1761541377 hasConceptScore W1761541377C4918238 @default.
- W1761541377 hasConceptScore W1761541377C54355233 @default.
- W1761541377 hasConceptScore W1761541377C86339819 @default.
- W1761541377 hasConceptScore W1761541377C86803240 @default.
- W1761541377 hasIssue "3" @default.
- W1761541377 hasLocation W17615413771 @default.
- W1761541377 hasLocation W17615413772 @default.
- W1761541377 hasLocation W17615413773 @default.
- W1761541377 hasOpenAccess W1761541377 @default.
- W1761541377 hasPrimaryLocation W17615413771 @default.
- W1761541377 hasRelatedWork W1568880892 @default.
- W1761541377 hasRelatedWork W1912770350 @default.
- W1761541377 hasRelatedWork W1993881548 @default.
- W1761541377 hasRelatedWork W2077166550 @default.
- W1761541377 hasRelatedWork W2099275218 @default.
- W1761541377 hasRelatedWork W2154835349 @default.
- W1761541377 hasRelatedWork W2156020179 @default.
- W1761541377 hasRelatedWork W2171277850 @default.
- W1761541377 hasRelatedWork W2386978125 @default.
- W1761541377 hasRelatedWork W381567666 @default.
- W1761541377 hasVolume "135" @default.
- W1761541377 isParatext "false" @default.
- W1761541377 isRetracted "false" @default.
- W1761541377 magId "1761541377" @default.
- W1761541377 workType "article" @default.