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• W1765129678 startingPage "893" @default.
• W1765129678 abstract "The icd gene of Escherichia coli, encoding isocitrate dehydrogenase, was shown to be expressed from two different promoters: the previously identified icd P1 and a newly detected second promoter, icd P2, whose expression is positively regulated by the catabolite repressor-activator protein Cra, formerly called FruR. In each case, we determined the mRNA start site by primer extension analysis of in vivo transcripts and examined the interaction of the icd control region with either RNA polymerase or Cra. We observed that (i) the Cra factor binds to and activates transcription from a site centered at position -76.5 within the icd P2 promoter region and (ii) three particular mutations in the C-terminal end of the alpha subunit of RNA polymerase (L262A, R265A, and N268A) considerably diminish transcription initiating from the icd P2 promoter, as shown by in vitro experiments performed in the presence of mutant RNA polymerases carrying Ala substitutions." @default.
• W1765129678 created "2016-06-24" @default.
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• W1765129678 date "1999-02-01" @default.
• W1765129678 modified "2023-10-16" @default.
• W1765129678 title "Cra-Dependent Transcriptional Activation of the <i>icd</i> Gene of <i>Escherichia coli</i>" @default.
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• W1765129678 doi "https://doi.org/10.1128/jb.181.3.893-898.1999" @default.
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