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- W1765627098 abstract "Integrated viral sequences and high sequence variability among badnaviruses complicates thedevelopment of specific reliable molecular detection tests for yam-infecting badnaviruses. ThusSerological techniques are of notable importance for routine testing and monitoring of these viruses.The major limiting factor to the use of serological techniques is the limited availability of antibodies.Rabbit polyclonal antibody was produced against a purified preparation of a yam-infecting badnavirusfrom Nigeria. Antibody titre was determined by Protein-A sandwich (PAS) enzyme-linkedimmunosorbent assay (ELISA). The antibody produced had a titre of 1:1280 in PAS-ELISA anddetected yam-infecting badnaviruses in infected yam leaves from Nigeria, Ghana, Benin and Togo. Thesuitability of the antibody for use in immunocapture polymerase chain reaction (IC-PCR) was evaluated.The antibody successfully trapped both Dioscorea alata bacilliform virus (DaBV) and Dioscoreasansibarensis bacilliform virus (DsBV) for IC-PCR detection. The antibody produced in this study willenhance certification of yam planting materials across West Africa and also facilitate the safeinternational movement of yam germplasm." @default.
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- W1765627098 date "2010-01-01" @default.
- W1765627098 modified "2023-09-22" @default.
- W1765627098 title "Production of Polyclonal Antibody Against an Isolate of Yam-infecting Badnavirus from Nigeria" @default.
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