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- W1767584057 abstract "Bacillus licheniformis is an organism of great scientific and industrial interest. The analysis of this important bacterium suffers from poor genetic accessibility. Therefore DNA transfer is a central problem for the construction of markerless deletion mutants in B. licheniformis DSM13. Due to the low natural competence of type strain DSM13 conjugative plasmid transfer is the method of choice for introduction of DNA. The pKVM-System was constructed for plasmid-based conjugative DNA-transfer to a variety of Bacillus species and used for strain improvement by introduction of small and large deletions. B. licheniformis H1, a close relative of B. licheniformis DSM13, possesses effective restriction-modification-systems. Therefore an in-vivo methylation system was established to create strain specific methylation patterns. This method was used for introduction of a deletion vector for markerless deletion in B. licheniformis H1. The PBSX-orthologous prophage, an inducible phage of B. licheniformis MW3, was deleted by homologous recombination. The mutant revealed less sensitivity against the phage inducible agent mitomycin C. However, no PBSX-like phage particles were detected. For growth on urea the urease gene cluster from B. licheniformis 9945A was transferred to B. licheniformis MW3, an urease-negative strain. Instability of the insertion was observed and no phenotype was detectable. The genome of B. licheniformis 9945A, a natural competent strain, was sequenced and analysed. Comparison to B. licheniformis DSM13 revealed a smaller amount of potential prophages. Three NRPS gene cluster were detected and similarities to genes of fengycin, bacitracin and lichenysin biosynthesis were observed." @default.
- W1767584057 created "2016-06-24" @default.
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- W1767584057 date "2010-07-29" @default.
- W1767584057 modified "2023-09-24" @default.
- W1767584057 title "Stammdesign in B. licheniformis" @default.
- W1767584057 hasPublicationYear "2010" @default.
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