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- W1767787079 startingPage "74" @default.
- W1767787079 abstract "Protein synthesis and degradation represent substantial costs during plant growth. To obtain a quantitative measure of the rate of protein synthesis and degradation, we supplied (13)CO2 to intact Arabidopsis (Arabidopsis thaliana) Columbia-0 plants and analyzed enrichment in free amino acids and in amino acid residues in protein during a 24-h pulse and 4-d chase. While many free amino acids labeled slowly and incompletely, alanine showed a rapid rise in enrichment in the pulse and a decrease in the chase. Enrichment in free alanine was used to correct enrichment in alanine residues in protein and calculate the rate of protein synthesis. The latter was compared with the relative growth rate to estimate the rate of protein degradation. The relative growth rate was estimated from sequential determination of fresh weight, sequential images of rosette area, and labeling of glucose in the cell wall. In an 8-h photoperiod, protein synthesis and cell wall synthesis were 3-fold faster in the day than at night, protein degradation was slow (3%-4% d(-1)), and flux to growth and degradation resulted in a protein half-life of 3.5 d. In the starchless phosphoglucomutase mutant at night, protein synthesis was further decreased and protein degradation increased, while cell wall synthesis was totally inhibited, quantitatively accounting for the inhibition of growth in this mutant. We also investigated the rates of protein synthesis and degradation during leaf development, during growth at high temperature, and compared synthesis rates of Rubisco large and small subunits of in the light and dark." @default.
- W1767787079 created "2016-06-24" @default.
- W1767787079 creator A5021262425 @default.
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- W1767787079 creator A5066445561 @default.
- W1767787079 creator A5080580916 @default.
- W1767787079 creator A5082852942 @default.
- W1767787079 date "2015-03-25" @default.
- W1767787079 modified "2023-10-17" @default.
- W1767787079 title "Quantifying Protein Synthesis and Degradation in Arabidopsis by Dynamic <sup>13</sup>CO<sub>2</sub> Labeling and Analysis of Enrichment in Individual Amino Acids in Their Free Pools and in Protein" @default.
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