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• W17690947 abstract "The development of fluorescence in situ hybridization (FISH) has led to the advancement of chromosome studies not only for physical mapping and genome analyses but also as a tool for evolutionary studies. Isolated repetitive DNA sequences have been useful cytological markers, but large-insert genomic libraries (e.g., bacterial artificial chromosomes [BACs], yeast artificial chromosomes [YACs], and cosmids) are being increasingly used to serve as probes for large segments of DNA across related genomes. Although FISH is usually applied to metaphase chromosomes, fiber FISH, a variation of FISH using extended DNA fibers, is now used to measure loci at the resolution of a few kilobases to compare orthologous genome segments across related genomes. A generalized set of protocols for chromosome preparation, FISH, and fiber FISH are presented; however, it is often necessary to experiment with techniques for different plant taxa for successful molecular cytogenetic studies." @default.
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• W17690947 date "2005-01-01" @default.
• W17690947 modified "2023-09-25" @default.
• W17690947 title "Preparation of Samples for Comparative Studies of Plant Chromosomes Using In Situ Hybridization Methods" @default.
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• W17690947 doi "https://doi.org/10.1016/s0076-6879(05)95024-6" @default.
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