FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1770666768> ?p ?o ?g. }

• W1770666768 endingPage "2079" @default.
• W1770666768 startingPage "2070" @default.
• W1770666768 abstract "In a previous report we showed that transcripts initiating from the late promoter of integrated polyoma plasmids could be detected at significant levels when neomycin resistance (neo) coding sequences were linked to this promoter. In this report we used chimeric plasmids that contain either a limited portion of the polyoma genome or deletions within the polyoma noncoding regulatory region to determine the sequence requirements for late promoter activity in this system. We observed no absolute requirement for either the polyoma early coding region or the origin of DNA replication for Neo-r colony formation. We were therefore able to independently assess the effects of deletions in the polyoma enhancer region on gene activity in both the early and late directions. We measured the ability of cells transfected with plasmids containing deletions in this region to form colonies in either semisolid or G418-containing medium under nonreplicative conditions. Our results indicate that either the PvuII 4 fragment, which contains the simian virus 40 core enhancer sequence, or a region from nucleotides 5099 to 5142, which contains the adenovirus type 5 E1A core enhancer sequence, can be deleted without significantly affecting gene expression in either direction. However, a deletion of nucleotides 5099 to 5172 reduced activities to similar extents in both directions, and a plasmid containing a larger deletion of nucleotides 5055 to 5182 showed a further reduction in activity. Although having no effect by itself, a second origin region deletion of nucleotides 5246 to 127 when present in these mutant backgrounds caused either a further reduction or elimination, respectively, of both G418 and agar colony-forming ability, suggesting the presence of an additional common regulatory element within this region. A comparison of 5' ends of neo transcripts present in cells transformed by these plasmids suggested that the reduction in activity was due to deletion of regulatory rather than structural elements of the late promoter. Our results indicate that the noncoding region of polyoma contains multiple complementing regulatory elements that control the level of both early and late gene expression." @default.
• W1770666768 created "2016-06-24" @default.
• W1770666768 creator A5015845099 @default.
• W1770666768 creator A5035617308 @default.
• W1770666768 creator A5091002644 @default.
• W1770666768 date "1985-08-01" @default.
• W1770666768 modified "2023-09-25" @default.
• W1770666768 title "Common regulatory elements control gene expression from polyoma early and late promoters in cells transformed by chimeric plasmids." @default.
• W1770666768 cites W1506041904 @default.
• W1770666768 cites W1549756639 @default.
• W1770666768 cites W1558516755 @default.
• W1770666768 cites W1566396044 @default.
• W1770666768 cites W1585046916 @default.
• W1770666768 cites W1793742390 @default.
• W1770666768 cites W1916807546 @default.
• W1770666768 cites W1917506099 @default.
• W1770666768 cites W1926153927 @default.
• W1770666768 cites W1937239515 @default.
• W1770666768 cites W1968244375 @default.
• W1770666768 cites W1970517901 @default.
• W1770666768 cites W1971420993 @default.
• W1770666768 cites W1973224141 @default.
• W1770666768 cites W1973537154 @default.
• W1770666768 cites W1977064370 @default.
• W1770666768 cites W1981042913 @default.
• W1770666768 cites W1983525206 @default.
• W1770666768 cites W1984513339 @default.
• W1770666768 cites W1989237565 @default.
• W1770666768 cites W1994564605 @default.
• W1770666768 cites W2018219360 @default.
• W1770666768 cites W2019590714 @default.
• W1770666768 cites W2027149534 @default.
• W1770666768 cites W2033013773 @default.
• W1770666768 cites W2035434814 @default.
• W1770666768 cites W2037412722 @default.
• W1770666768 cites W2038218774 @default.
• W1770666768 cites W2045583364 @default.
• W1770666768 cites W2047126923 @default.
• W1770666768 cites W2052513192 @default.
• W1770666768 cites W2053956877 @default.
• W1770666768 cites W2054073262 @default.
• W1770666768 cites W2055859544 @default.
• W1770666768 cites W2056618475 @default.
• W1770666768 cites W2060730709 @default.
• W1770666768 cites W2062408930 @default.
• W1770666768 cites W2062417572 @default.
• W1770666768 cites W2066352920 @default.
• W1770666768 cites W2067956561 @default.
• W1770666768 cites W2074522847 @default.
• W1770666768 cites W2082108715 @default.
• W1770666768 cites W2084100792 @default.
• W1770666768 cites W2088622701 @default.
• W1770666768 cites W2090628750 @default.
• W1770666768 cites W2092216600 @default.
• W1770666768 cites W2118929543 @default.
• W1770666768 cites W2120033918 @default.
• W1770666768 cites W2134078619 @default.
• W1770666768 cites W2135005590 @default.
• W1770666768 cites W2136347509 @default.
• W1770666768 cites W2141648783 @default.
• W1770666768 cites W2142737618 @default.
• W1770666768 cites W2157589212 @default.
• W1770666768 cites W2158290831 @default.
• W1770666768 cites W2165148733 @default.
• W1770666768 cites W2170398046 @default.
• W1770666768 cites W2316008018 @default.
• W1770666768 cites W2320444745 @default.
• W1770666768 cites W72100183 @default.
• W1770666768 doi "https://doi.org/10.1128/mcb.5.8.2070" @default.
• W1770666768 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/366925" @default.
• W1770666768 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3018549" @default.
• W1770666768 hasPublicationYear "1985" @default.
• W1770666768 type Work @default.
• W1770666768 sameAs 1770666768 @default.
• W1770666768 citedByCount "24" @default.
• W1770666768 crossrefType "journal-article" @default.
• W1770666768 hasAuthorship W1770666768A5015845099 @default.
• W1770666768 hasAuthorship W1770666768A5035617308 @default.
• W1770666768 hasAuthorship W1770666768A5091002644 @default.
• W1770666768 hasBestOaLocation W17706667681 @default.
• W1770666768 hasConcept C101762097 @default.
• W1770666768 hasConcept C104317684 @default.
• W1770666768 hasConcept C111936080 @default.
• W1770666768 hasConcept C150194340 @default.
• W1770666768 hasConcept C153911025 @default.
• W1770666768 hasConcept C21592294 @default.
• W1770666768 hasConcept C22744801 @default.
• W1770666768 hasConcept C54009773 @default.
• W1770666768 hasConcept C54355233 @default.
• W1770666768 hasConcept C552990157 @default.
• W1770666768 hasConcept C58337764 @default.
• W1770666768 hasConcept C84148353 @default.
• W1770666768 hasConcept C86803240 @default.
• W1770666768 hasConcept C91779695 @default.
• W1770666768 hasConceptScore W1770666768C101762097 @default.
• W1770666768 hasConceptScore W1770666768C104317684 @default.
• W1770666768 hasConceptScore W1770666768C111936080 @default.
• W1770666768 hasConceptScore W1770666768C150194340 @default.

• next