Matches in SemOpenAlex for { <https://semopenalex.org/work/W1771027239> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1771027239 endingPage "648" @default.
- W1771027239 startingPage "635" @default.
- W1771027239 abstract "This chapter describes the instrumentation, analytic methods, and fluorescent probes for measurement of diffusion in cells by photobleaching methods. Fluorescence recovery after photobleaching is a useful experimental method for measurement of the translational diffusion of fluorophores and fluorescently labeled macromolecules in cellular compartments. Fluorescent labels are targeted to cellular aqueous or membrane compartments by transfection methods (for the green fluorescent protein) or are incorporated by incubation or microinjection methods. The instrumentation for photobleaching consists of a laser/light source to bleach and probe a defined sample volume, a fluorescence microscope, and a detector. Photobleaching measurements of cultured cells grown on transparent supports are done with a temperature-regulated perfusion chamber to immobilize the cell layer and allow changes in perfusate solution composition. An objective lens heater is used to prevent temperature differences between the sample and immersion objectives. Fluorescent probes are microinjected into fluid droplets secreted onto the airway surface under oil, and spot photobleaching is carried out with an approximately parallel laser beam produced by a low-numerical aperture lens." @default.
- W1771027239 created "2016-06-24" @default.
- W1771027239 creator A5042356327 @default.
- W1771027239 date "2003-01-01" @default.
- W1771027239 modified "2023-10-13" @default.
- W1771027239 title "[28] Diffusion in cells measured by fluorescence recovery after photobleaching" @default.
- W1771027239 cites W1530729226 @default.
- W1771027239 cites W1853541631 @default.
- W1771027239 cites W1977056005 @default.
- W1771027239 cites W1993645665 @default.
- W1771027239 cites W1997472848 @default.
- W1771027239 cites W2002478108 @default.
- W1771027239 cites W2006168822 @default.
- W1771027239 cites W2017934591 @default.
- W1771027239 cites W2023193053 @default.
- W1771027239 cites W2024886150 @default.
- W1771027239 cites W2026597183 @default.
- W1771027239 cites W2026878536 @default.
- W1771027239 cites W2026896701 @default.
- W1771027239 cites W2039190086 @default.
- W1771027239 cites W2047273078 @default.
- W1771027239 cites W2049049344 @default.
- W1771027239 cites W2051979309 @default.
- W1771027239 cites W2056204193 @default.
- W1771027239 cites W2067720692 @default.
- W1771027239 cites W2070992372 @default.
- W1771027239 cites W2080661489 @default.
- W1771027239 cites W2110706129 @default.
- W1771027239 cites W2113092217 @default.
- W1771027239 cites W2116043125 @default.
- W1771027239 cites W2119006743 @default.
- W1771027239 cites W2125577771 @default.
- W1771027239 cites W2128341772 @default.
- W1771027239 cites W2132629429 @default.
- W1771027239 cites W2139125300 @default.
- W1771027239 cites W2148303154 @default.
- W1771027239 cites W2158587991 @default.
- W1771027239 cites W2167949866 @default.
- W1771027239 doi "https://doi.org/10.1016/s0076-6879(03)60132-1" @default.
- W1771027239 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12622172" @default.
- W1771027239 hasPublicationYear "2003" @default.
- W1771027239 type Work @default.
- W1771027239 sameAs 1771027239 @default.
- W1771027239 citedByCount "49" @default.
- W1771027239 countsByYear W17710272392012 @default.
- W1771027239 countsByYear W17710272392013 @default.
- W1771027239 countsByYear W17710272392014 @default.
- W1771027239 countsByYear W17710272392015 @default.
- W1771027239 countsByYear W17710272392016 @default.
- W1771027239 countsByYear W17710272392018 @default.
- W1771027239 countsByYear W17710272392019 @default.
- W1771027239 countsByYear W17710272392020 @default.
- W1771027239 countsByYear W17710272392021 @default.
- W1771027239 countsByYear W17710272392022 @default.
- W1771027239 crossrefType "book-chapter" @default.
- W1771027239 hasAuthorship W1771027239A5042356327 @default.
- W1771027239 hasConcept C120665830 @default.
- W1771027239 hasConcept C121332964 @default.
- W1771027239 hasConcept C12554922 @default.
- W1771027239 hasConcept C147080431 @default.
- W1771027239 hasConcept C169274487 @default.
- W1771027239 hasConcept C185592680 @default.
- W1771027239 hasConcept C192562407 @default.
- W1771027239 hasConcept C26659971 @default.
- W1771027239 hasConcept C31332276 @default.
- W1771027239 hasConcept C67649825 @default.
- W1771027239 hasConcept C86803240 @default.
- W1771027239 hasConcept C91881484 @default.
- W1771027239 hasConceptScore W1771027239C120665830 @default.
- W1771027239 hasConceptScore W1771027239C121332964 @default.
- W1771027239 hasConceptScore W1771027239C12554922 @default.
- W1771027239 hasConceptScore W1771027239C147080431 @default.
- W1771027239 hasConceptScore W1771027239C169274487 @default.
- W1771027239 hasConceptScore W1771027239C185592680 @default.
- W1771027239 hasConceptScore W1771027239C192562407 @default.
- W1771027239 hasConceptScore W1771027239C26659971 @default.
- W1771027239 hasConceptScore W1771027239C31332276 @default.
- W1771027239 hasConceptScore W1771027239C67649825 @default.
- W1771027239 hasConceptScore W1771027239C86803240 @default.
- W1771027239 hasConceptScore W1771027239C91881484 @default.
- W1771027239 hasLocation W17710272391 @default.
- W1771027239 hasLocation W17710272392 @default.
- W1771027239 hasOpenAccess W1771027239 @default.
- W1771027239 hasPrimaryLocation W17710272391 @default.
- W1771027239 hasRelatedWork W1493190780 @default.
- W1771027239 hasRelatedWork W1502469574 @default.
- W1771027239 hasRelatedWork W2009654376 @default.
- W1771027239 hasRelatedWork W2042460426 @default.
- W1771027239 hasRelatedWork W2053704624 @default.
- W1771027239 hasRelatedWork W2101336703 @default.
- W1771027239 hasRelatedWork W3133220949 @default.
- W1771027239 hasRelatedWork W3144685119 @default.
- W1771027239 hasRelatedWork W4241546789 @default.
- W1771027239 hasRelatedWork W4284977144 @default.
- W1771027239 isParatext "false" @default.
- W1771027239 isRetracted "false" @default.
- W1771027239 magId "1771027239" @default.
- W1771027239 workType "book-chapter" @default.