Matches in SemOpenAlex for { <https://semopenalex.org/work/W1772202354> ?p ?o ?g. }
- W1772202354 endingPage "182" @default.
- W1772202354 startingPage "172" @default.
- W1772202354 abstract "The structure and lipid thermotropic transitions of highly purified lipophorin of Triatoma infestans were examined by several techniques: steady-state fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene (DPH), cis-parinaric acid (cis-PnA) and trans-parinaric acid (trans-PnA), light scattering fluorescence energy transfer between the lipophorin tryptophan residues and the bound chromophores, DPH, trans-parinaric acid cis-parinaric acid, gel electrophoresis, and gel filtration. Fluorescence polarization of PnAs and DPH revealed a reversible lipid thermotropic transition in intact lipophorin at about 20 degrees C and 18 degrees C, respectively. In lipophorin, lipid dispersion fluorescence polarization of DPH detected a lipid transition approximately at 20 degrees C, while trans-PnA showed a gel phase formation at a temperature below 30 degrees C. Similar experiments in which trans-PnA was incorporated into diacylglycerols and phospholipids extracted from the lipophorin revealed gel phase formation below 30 degrees C and 24 degrees C, respectively. Light scattering measurements showed that lipophorin particles aggregate irreversibly at 45 degrees C, increasing the molecular weight, as determined by gel filtration on Sephacryl S-300, from 740,000 to values larger than 1,500,000. The particle aggregation did not change the physical properties of the lipophorin studied by fluorescence polarization, indicating that the aggregation is apparently a non-denaturing process. Energy transfer between the lipophorin tryptophans and the bound chromophores cis-PnA, trans-PnA, and DPA revealed a different location of the fluorescent probes within the lipophorin. Temperature-dependence on the energy transfer efficiency for all probes confirmed a change in the ordering of the lipophorin lipids at 24 degrees C." @default.
- W1772202354 created "2016-06-24" @default.
- W1772202354 creator A5004925764 @default.
- W1772202354 creator A5009095648 @default.
- W1772202354 creator A5045628575 @default.
- W1772202354 date "1988-02-01" @default.
- W1772202354 modified "2023-10-08" @default.
- W1772202354 title "Lipid thermotropic transitions in Triatoma infestans lipophorin." @default.
- W1772202354 cites W1526385127 @default.
- W1772202354 cites W1533773615 @default.
- W1772202354 cites W1775749144 @default.
- W1772202354 cites W1964679056 @default.
- W1772202354 cites W1966313979 @default.
- W1772202354 cites W1972234231 @default.
- W1772202354 cites W1972550565 @default.
- W1772202354 cites W1972890152 @default.
- W1772202354 cites W1978252847 @default.
- W1772202354 cites W1978265259 @default.
- W1772202354 cites W1980408280 @default.
- W1772202354 cites W1985342306 @default.
- W1772202354 cites W1990999961 @default.
- W1772202354 cites W1998312071 @default.
- W1772202354 cites W2001825873 @default.
- W1772202354 cites W2002352808 @default.
- W1772202354 cites W2002660914 @default.
- W1772202354 cites W2006165901 @default.
- W1772202354 cites W2017104656 @default.
- W1772202354 cites W2021007036 @default.
- W1772202354 cites W2024990576 @default.
- W1772202354 cites W2025830649 @default.
- W1772202354 cites W2026880510 @default.
- W1772202354 cites W2027720734 @default.
- W1772202354 cites W2040391473 @default.
- W1772202354 cites W2042436691 @default.
- W1772202354 cites W2047556375 @default.
- W1772202354 cites W2051446091 @default.
- W1772202354 cites W2053400975 @default.
- W1772202354 cites W2059953019 @default.
- W1772202354 cites W2060167427 @default.
- W1772202354 cites W2074484338 @default.
- W1772202354 cites W2074549463 @default.
- W1772202354 cites W2074618866 @default.
- W1772202354 cites W2083457891 @default.
- W1772202354 cites W2083798431 @default.
- W1772202354 cites W2089253866 @default.
- W1772202354 cites W2090178654 @default.
- W1772202354 cites W2131970139 @default.
- W1772202354 cites W2132741431 @default.
- W1772202354 cites W2146521475 @default.
- W1772202354 cites W2148529609 @default.
- W1772202354 cites W2163850310 @default.
- W1772202354 cites W2168526937 @default.
- W1772202354 cites W2200436717 @default.
- W1772202354 cites W4299564447 @default.
- W1772202354 doi "https://doi.org/10.1016/s0022-2275(20)38544-8" @default.
- W1772202354 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3284956" @default.
- W1772202354 hasPublicationYear "1988" @default.
- W1772202354 type Work @default.
- W1772202354 sameAs 1772202354 @default.
- W1772202354 citedByCount "13" @default.
- W1772202354 countsByYear W17722023542013 @default.
- W1772202354 crossrefType "journal-article" @default.
- W1772202354 hasAuthorship W1772202354A5004925764 @default.
- W1772202354 hasAuthorship W1772202354A5009095648 @default.
- W1772202354 hasAuthorship W1772202354A5045628575 @default.
- W1772202354 hasBestOaLocation W17722023541 @default.
- W1772202354 hasConcept C105702510 @default.
- W1772202354 hasConcept C136764020 @default.
- W1772202354 hasConcept C178790620 @default.
- W1772202354 hasConcept C185592680 @default.
- W1772202354 hasConcept C22064058 @default.
- W1772202354 hasConcept C2776468184 @default.
- W1772202354 hasConcept C2777625466 @default.
- W1772202354 hasConcept C2778554237 @default.
- W1772202354 hasConcept C2779538911 @default.
- W1772202354 hasConcept C2780394580 @default.
- W1772202354 hasConcept C2994273327 @default.
- W1772202354 hasConcept C41008148 @default.
- W1772202354 hasConcept C44280652 @default.
- W1772202354 hasConcept C71928629 @default.
- W1772202354 hasConcept C86803240 @default.
- W1772202354 hasConcept C90856448 @default.
- W1772202354 hasConceptScore W1772202354C105702510 @default.
- W1772202354 hasConceptScore W1772202354C136764020 @default.
- W1772202354 hasConceptScore W1772202354C178790620 @default.
- W1772202354 hasConceptScore W1772202354C185592680 @default.
- W1772202354 hasConceptScore W1772202354C22064058 @default.
- W1772202354 hasConceptScore W1772202354C2776468184 @default.
- W1772202354 hasConceptScore W1772202354C2777625466 @default.
- W1772202354 hasConceptScore W1772202354C2778554237 @default.
- W1772202354 hasConceptScore W1772202354C2779538911 @default.
- W1772202354 hasConceptScore W1772202354C2780394580 @default.
- W1772202354 hasConceptScore W1772202354C2994273327 @default.
- W1772202354 hasConceptScore W1772202354C41008148 @default.
- W1772202354 hasConceptScore W1772202354C44280652 @default.
- W1772202354 hasConceptScore W1772202354C71928629 @default.
- W1772202354 hasConceptScore W1772202354C86803240 @default.
- W1772202354 hasConceptScore W1772202354C90856448 @default.