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- W1772422493 abstract "Conjugations of DNA with chitosans 15 kD (ch-15), 100 kD (ch-100) and 200 kD (ch-200) were investigated in aqueous solution at pH 5.5–6.5. Multiple spectroscopic methods and atomic force microscopy (AFM) were used to locate the chitosan binding sites and the effect of polymer conjugation on DNA compaction and particle formation. Structural analysis showed that chitosan–DNA conjugation is mainly via electrostatic interactions through polymer cationic charged NH2 and negatively charged backbone phosphate groups. As polymer size increases major DNA compaction and particle formation occurs. At high chitosan concentration major DNA structural changes observed indicating a partial B to A-DNA conformational transition." @default.
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- W1772422493 date "2016-02-01" @default.
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- W1772422493 title "Microscopic and spectroscopic analysis of chitosan–DNA conjugates" @default.
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- W1772422493 doi "https://doi.org/10.1016/j.carbpol.2015.09.080" @default.
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