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• W17741392 abstract "The main subject of this thesis is the Sulphate Permease (SulP) protein family that includes more than two hundred members, identified in archaea, bacteria, fungi, plants and animals. Many of these proteins have been functionally characterized: most are anion exchangers or transporters with different substrate specificities and distinct mechanism of action (Saier et al., 1999). In mammals, the SulP family, known as Solute Linked Carrier 26 (SLC26), is composed of eleven members with important roles in normal physiology (Mount and Romero, 2004).The SulP proteins show a similar structural organization: a hydrophobic central core, which includes ten or twelve membrane helixes, and a less conserved C-terminal cytoplasmic portion that includes a STAS domain (Sulphate Transporter and Anti-Sigma factor antagonist domain). Despite the functional role of the STAS domain is still unclear, it appears to be crucial for the regulation of the transport activity (Ko et al., 2004; Zheng et al., 2005; Shibagaki and Grossman, 2006). Its fundamental role is also underlined by the fact that mutations that alter this domain in the SLC26 family can cause loss of function, resulting in serious genetic diseases, like diastrophic dysplasia or Pendred syndrome (Dawson and Markovich, 2005). No three-dimensional structure of STAS domains or full-length sequences is available for any SulP anion transporter. One part of the work was focused on the production of different forms of the STAS domain from different species, for the biophysical and structural characterization. Another part of the SulP project was performed at the Johann Wolfgang Goethe University of Frankfurt (Germany) and aimed at the production of some full-length SulP proteins, by a cell-free expression system, an emerging technique for the large-scale production of membrane proteins. In the last year, I was also involved in the crystallographic study of the Green Fluorescence Protein mutant, GFPmut2, in collaboration with Prof. Stefano Bettati of the University of Parma (Italy). The main aim of this work was the elucidation of the structural basis of the spectroscopic properties of this mutant, in particular with respect to changes in pH. The GFP chromophore can, in fact, exist either in a protonated or deprotonated state, with distinct spectral properties (Tsien, 1998). In a previous spectroscopic characterization, GFPmut2 (Ser65Ala, Val68Leu, Ser72Ala) was found more sensitive than the wild type GFP to pH changes in the physiological range (Chirico et al., 2002). The structures of GFPmut2 at pH 6 and pH 9 were determined at around 1.6 A resolution, allowing the correlation between the spectral and structural properties." @default.
• W17741392 created "2016-06-24" @default.
• W17741392 creator A5078011667 @default.
• W17741392 date "2009-02-02" @default.
• W17741392 modified "2023-09-27" @default.
• W17741392 title "Production and characterization of SulP anion transporters" @default.
• W17741392 cites W1494253611 @default.
• W17741392 cites W1499587699 @default.
• W17741392 cites W1519680742 @default.
• W17741392 cites W1521207244 @default.
• W17741392 cites W1546187595 @default.
• W17741392 cites W1549745968 @default.
• W17741392 cites W1554225425 @default.
• W17741392 cites W1588654475 @default.
• W17741392 cites W1666755687 @default.
• W17741392 cites W1901461509 @default.
• W17741392 cites W1934714969 @default.
• W17741392 cites W1964455053 @default.
• W17741392 cites W1965632620 @default.
• W17741392 cites W1969094022 @default.
• W17741392 cites W1970928753 @default.
• W17741392 cites W1972872799 @default.
• W17741392 cites W1973567631 @default.
• W17741392 cites W1974243596 @default.
• W17741392 cites W1974737961 @default.
• W17741392 cites W1981531296 @default.
• W17741392 cites W1983544779 @default.
• W17741392 cites W1983547144 @default.
• W17741392 cites W1986733224 @default.
• W17741392 cites W1989615447 @default.
• W17741392 cites W1992714668 @default.
• W17741392 cites W1993648229 @default.
• W17741392 cites W1994074744 @default.
• W17741392 cites W1994102565 @default.
• W17741392 cites W1997665931 @default.
• W17741392 cites W1999602579 @default.
• W17741392 cites W2001641653 @default.
• W17741392 cites W2001741929 @default.
• W17741392 cites W2002360833 @default.
• W17741392 cites W2005096891 @default.
• W17741392 cites W2006260688 @default.
• W17741392 cites W2006685379 @default.
• W17741392 cites W2006991314 @default.
• W17741392 cites W2010526046 @default.
• W17741392 cites W2013412105 @default.
• W17741392 cites W2014293907 @default.
• W17741392 cites W2014432781 @default.
• W17741392 cites W2014781338 @default.
• W17741392 cites W2015871068 @default.
• W17741392 cites W2017923651 @default.
• W17741392 cites W2018593981 @default.
• W17741392 cites W2020991261 @default.
• W17741392 cites W2023794896 @default.
• W17741392 cites W2023881054 @default.
• W17741392 cites W2028267773 @default.
• W17741392 cites W2030239671 @default.
• W17741392 cites W2030413354 @default.
• W17741392 cites W2030695915 @default.
• W17741392 cites W2032579724 @default.
• W17741392 cites W2033670275 @default.
• W17741392 cites W2033726591 @default.
• W17741392 cites W2035128899 @default.
• W17741392 cites W2035966657 @default.
• W17741392 cites W2036169850 @default.
• W17741392 cites W2036489477 @default.
• W17741392 cites W2036742051 @default.
• W17741392 cites W2038717281 @default.
• W17741392 cites W2043415327 @default.
• W17741392 cites W2044812677 @default.
• W17741392 cites W2048714645 @default.
• W17741392 cites W2048994244 @default.
• W17741392 cites W2052512546 @default.
• W17741392 cites W2053264108 @default.
• W17741392 cites W2053921060 @default.
• W17741392 cites W2055144172 @default.
• W17741392 cites W2056399837 @default.
• W17741392 cites W2061104342 @default.
• W17741392 cites W2061868139 @default.
• W17741392 cites W2062876104 @default.
• W17741392 cites W2065969023 @default.
• W17741392 cites W2066990884 @default.
• W17741392 cites W2066996551 @default.
• W17741392 cites W2070044371 @default.
• W17741392 cites W2073256112 @default.
• W17741392 cites W2073282504 @default.
• W17741392 cites W2074276238 @default.
• W17741392 cites W2075437668 @default.
• W17741392 cites W2079233479 @default.
• W17741392 cites W2080803336 @default.
• W17741392 cites W2084312697 @default.
• W17741392 cites W2085885797 @default.
• W17741392 cites W2086057760 @default.
• W17741392 cites W2089035094 @default.
• W17741392 cites W2093163087 @default.
• W17741392 cites W2095871457 @default.
• W17741392 cites W2101871915 @default.
• W17741392 cites W2104950518 @default.
• W17741392 cites W2111340047 @default.
• W17741392 cites W2111513328 @default.
• W17741392 cites W2115904275 @default.

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