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- W1775394094 abstract "Research Article1 October 1984free access The 43-K protein, v1, associated with acetylcholine receptor containing membrane fragments is an actin-binding protein. J.H. Walker J.H. Walker Search for more papers by this author C.M. Boustead C.M. Boustead Search for more papers by this author V. Witzemann V. Witzemann Search for more papers by this author J.H. Walker J.H. Walker Search for more papers by this author C.M. Boustead C.M. Boustead Search for more papers by this author V. Witzemann V. Witzemann Search for more papers by this author Author Information J.H. Walker, C.M. Boustead and V. Witzemann The EMBO Journal (1984)3:2287-2290https://doi.org/10.1002/j.1460-2075.1984.tb02127.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Acetylcholine receptor enriched membrane fragments were obtained from the electric organs of Torpedo marmorata. The purified membrane fragments contained several proteins in addition to the acetylcholine receptor subunits. One of these was shown to be actin by means of immune blotting with a monoclonal antibody. Brief treatment of the membranes with pH 11.0 buffer removed actin and the other non-receptor proteins including the receptor-associated 43 000 mol. wt. polypeptide. This polypeptide was shown to bind actin after transferring the proteins from one- and two-dimensional polyacrylamide gels to nitrocellulose paper and incubating the nitrocellulose blots with actin. Specifically bound actin was demonstrated using the monoclonal antibodies to actin. No calcium or calmodulin dependency of binding was observed. The findings suggest that the 43 000 mol. wt. polypeptide is a link between the membrane-bound acetylcholine receptor and the cytoskeleton. Previous ArticleNext Article Volume 3Issue 101 October 1984In this issue RelatedDetailsLoading ..." @default.
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- W1775394094 date "1984-10-01" @default.
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- W1775394094 title "The 43-K protein, v1, associated with acetylcholine receptor containing membrane fragments is an actin-binding protein." @default.
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- W1775394094 doi "https://doi.org/10.1002/j.1460-2075.1984.tb02127.x" @default.
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