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- W1775671103 abstract "Viral particles purified from species of the protozoan parasite Leishmania braziliensis subsp. guyanensis by centrifugation in CsCl gradients were examined for the presence of viral polymerase. We demonstrated that RNA-dependent RNA polymerase is associated with viral particles. Viral transcription was studied in vitro with pulse-chase experiments and by assaying the RNase sensitivity of the viral transcripts. Viral polymerase synthesized full-length transcripts within 1 h. Double-strained, genome-length, and single-stranded RNAs were produced in this system. The nature of the RNA extracted from virions was also tested by RNase protection assays; both single-stranded and double-stranded RNAs were found." @default.
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- W1775671103 date "1990-08-01" @default.
- W1775671103 modified "2023-09-27" @default.
- W1775671103 title "RNA polymerase activity is associated with viral particles isolated from Leishmania braziliensis subsp. guyanensis" @default.
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- W1775671103 doi "https://doi.org/10.1128/jvi.64.8.3712-3715.1990" @default.
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