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- W1776304336 abstract "We present droplet adaptive laboratory evolution (DrALE), a directed evolution method used to improve industrial enzyme producing microorganisms for e.g. feedstock digestion. DrALE is based linking a desired phenotype to growth rate allowing only desired cells to proliferate. Single cells are confined in microfluidic droplets to prevent the phenotype, e.g. secreted enzymes, from leaking between cells. The method was benchmarked against and found to significantly outperform conventional adaptive laboratory evolution (ALE) in enriching enzyme producing cells. It was furthermore applied to enrich a whole-genome mutated library of yeast cells for α-amylase activity." @default.
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- W1776304336 date "2015-01-01" @default.
- W1776304336 modified "2023-09-27" @default.
- W1776304336 title "Micro-droplet based directed evolution outperforms conventional laboratory evolution" @default.
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