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- W1777466114 abstract "This chapter reviews a macromolecular delivery technique that employs cells that express a potent membrane fusion protein, the influenza virus hemagglutinin (HA). Briefly, the protein or nucleic acid of interest is loaded into either red blood cells (RBC) or liposomes. After binding of the delivery vesicles to the HA-expressing cells, fusion is induced by briefly treating the cultures at pH 4.8. The cells are then returned to normal growth medium. As a result of the fusion step, the contents of the RBC or liposomes are delivered into the cell cytoplasm. This chapter discusses the methods for generating HA-expressing cell lines, for loading RBC and liposomes, and for fusing the delivery vesicles to target cells. The chapter evaluates the advantages and disadvantages of this method. There are several advantages to the technique: (1) nearly all of the cells in a population receive macromolecules, (2) large numbers of molecules can be delivered per cell (3) the same general approach and recipient cells can be used for the delivery of proteins, nucleic acids, and other substances. A limitation of the technique is the need to obtain cells that express high levels of the HA. However, several HA-expressing cell lines suitable for delivery are currently available." @default.
- W1777466114 created "2016-06-24" @default.
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- W1777466114 date "1989-01-01" @default.
- W1777466114 modified "2023-10-18" @default.
- W1777466114 title "Chapter 9 Delivery of Macromolecules into Cells Expressing a Viral Membrane Fusion Protein" @default.
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- W1777466114 doi "https://doi.org/10.1016/s0091-679x(08)61607-6" @default.
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