Matches in SemOpenAlex for { <https://semopenalex.org/work/W177768807> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W177768807 abstract "Dengue viral RNA polymerase replicates its positive single-stranded RNA genome in two phases: de novo initiation and elongation. The slow and inefficient initiation during replication masks the elongation mode. The aim of this work was to develop methodology to characterize the mechanism of elongation. Formation of the elongation complex required preincubation of the enzyme with a double stranded RNA (primer/template) for 1 hour at 37 °C in the presence of magnesium. The elongation complex incorporated a correct nucleotide at a rate of ~20 s−1. The fidelity of the polymerase, ~ 2 x 10−5, was determined by measuring the specificity of incorporation of correct and incorrect nucleotides. The reaction pathway comprised of enzyme and RNA binding followed by nucleotide binding and incorporation was studied by transient kinetic methods. The microscopic rate constants for each step of the pathway were obtained by global data fitting and computer simulation." @default.
- W177768807 created "2016-06-24" @default.
- W177768807 creator A5008946883 @default.
- W177768807 creator A5016607393 @default.
- W177768807 creator A5044383613 @default.
- W177768807 creator A5054934658 @default.
- W177768807 date "2010-04-01" @default.
- W177768807 modified "2023-10-17" @default.
- W177768807 title "Transient kinetic analysis of the elongation mode of Dengue Virus RNA polymerase domain" @default.
- W177768807 doi "https://doi.org/10.1096/fasebj.24.1_supplement.lb75" @default.
- W177768807 hasPublicationYear "2010" @default.
- W177768807 type Work @default.
- W177768807 sameAs 177768807 @default.
- W177768807 citedByCount "0" @default.
- W177768807 crossrefType "journal-article" @default.
- W177768807 hasAuthorship W177768807A5008946883 @default.
- W177768807 hasAuthorship W177768807A5016607393 @default.
- W177768807 hasAuthorship W177768807A5044383613 @default.
- W177768807 hasAuthorship W177768807A5054934658 @default.
- W177768807 hasConcept C104317684 @default.
- W177768807 hasConcept C112950240 @default.
- W177768807 hasConcept C12554922 @default.
- W177768807 hasConcept C153911025 @default.
- W177768807 hasConcept C178790620 @default.
- W177768807 hasConcept C181199279 @default.
- W177768807 hasConcept C185592680 @default.
- W177768807 hasConcept C191897082 @default.
- W177768807 hasConcept C192562407 @default.
- W177768807 hasConcept C2776449523 @default.
- W177768807 hasConcept C2777563447 @default.
- W177768807 hasConcept C33947775 @default.
- W177768807 hasConcept C512185932 @default.
- W177768807 hasConcept C55493867 @default.
- W177768807 hasConcept C67705224 @default.
- W177768807 hasConcept C82381507 @default.
- W177768807 hasConcept C86803240 @default.
- W177768807 hasConceptScore W177768807C104317684 @default.
- W177768807 hasConceptScore W177768807C112950240 @default.
- W177768807 hasConceptScore W177768807C12554922 @default.
- W177768807 hasConceptScore W177768807C153911025 @default.
- W177768807 hasConceptScore W177768807C178790620 @default.
- W177768807 hasConceptScore W177768807C181199279 @default.
- W177768807 hasConceptScore W177768807C185592680 @default.
- W177768807 hasConceptScore W177768807C191897082 @default.
- W177768807 hasConceptScore W177768807C192562407 @default.
- W177768807 hasConceptScore W177768807C2776449523 @default.
- W177768807 hasConceptScore W177768807C2777563447 @default.
- W177768807 hasConceptScore W177768807C33947775 @default.
- W177768807 hasConceptScore W177768807C512185932 @default.
- W177768807 hasConceptScore W177768807C55493867 @default.
- W177768807 hasConceptScore W177768807C67705224 @default.
- W177768807 hasConceptScore W177768807C82381507 @default.
- W177768807 hasConceptScore W177768807C86803240 @default.
- W177768807 hasIssue "S1" @default.
- W177768807 hasLocation W1777688071 @default.
- W177768807 hasOpenAccess W177768807 @default.
- W177768807 hasPrimaryLocation W1777688071 @default.
- W177768807 hasRelatedWork W1989179485 @default.
- W177768807 hasRelatedWork W2002878301 @default.
- W177768807 hasRelatedWork W2062114156 @default.
- W177768807 hasRelatedWork W2086527685 @default.
- W177768807 hasRelatedWork W2088467414 @default.
- W177768807 hasRelatedWork W2403448847 @default.
- W177768807 hasRelatedWork W24515824 @default.
- W177768807 hasRelatedWork W2895689929 @default.
- W177768807 hasRelatedWork W3119203274 @default.
- W177768807 hasRelatedWork W3119681108 @default.
- W177768807 hasVolume "24" @default.
- W177768807 isParatext "false" @default.
- W177768807 isRetracted "false" @default.
- W177768807 magId "177768807" @default.
- W177768807 workType "article" @default.