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- W1777974595 abstract "Publisher Summary This chapter focuses on the use of the absorbance and fluorescence of light to learn about structures and dynamics of biological macromolecules. Native states of proteins and nucleic acids can be distinguished from denatured states by measurement of the absorbance of unpolarized light. Fluorescence adds a very powerful set of spectroscopic parameters to relate to structure and dynamics. The excitation spectrum (the absorption spectrum which leads to fluorescence at a chosen wavelength) and the fluorescence emission spectrum can identify and characterize fluorophores in complex mixtures. Time-resolved fluorescence spectra provide greater discrimination by introducing time as another variable. Methods that use ultraviolet and visible light to learn about structures, dynamics, and therefore functions of biological molecules are presented in this chapter. The general theories are given, experimental methods are described, and specific applications are given to provide a good understanding of the wide range of methods available." @default.
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- W1777974595 date "1995-01-01" @default.
- W1777974595 modified "2023-09-26" @default.
- W1777974595 title "[2] Optical spectroscopy: General principles and overview" @default.
- W1777974595 doi "https://doi.org/10.1016/0076-6879(95)46004-7" @default.
- W1777974595 hasPublicationYear "1995" @default.
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