FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1780521159> ?p ?o ?g. }

• W1780521159 endingPage "1695" @default.
• W1780521159 startingPage "1685" @default.
• W1780521159 abstract "All-trans retinoic acid (ATRA) is one of the first line agents in differentiation therapy for acute promyelocytic leukemia (APL). However, drug resistance is a major problem influencing the efficacy of ATRA. Identification of mechanisms of ATRA resistance are urgenly needed. In the present study, we found that expression of C/EBPα, an important transcription factor for myeloid differentiation, was significantly suppressed in ATRA resistant APL cell line NB4-R1 compared with ATRA sensitive NB4 cells. Moreover, two forms of C/EBPα were unequally suppressed in NB4-R1 cells. Suppression of the full-length form P42 was more pronounced than the truncated form P30. Inhibition of PI3K/Akt/mTOR pathway was also observed in NB4-R1 cells. Moreover, C/EBPα expression was reduced by PI3K inhibitor LY294002 and mTOR inhibitor RAD001 in NB4 cells, suggesting that inactivation of the PI3K/Akt/mTOR pathway was responsible for C/EBPα suppression in APL cells. We restored C/EBPα P42 and P30 by lentivirus vectors in NB4-R1 cells, respectively, and found C/EBPα P42, but not P30, could increase CD11b, CD14, G-CSFR and GM-CSFR expression, which indicated the occurrence of myeloid differentiation. Further upregulating of CD11b expression and differential morphological changes were found in NB4-R1 cells with restored C/EBPα P42 after ATRA treatment. However, CD11b expression and differential morphological changes could not be induced by ATRA in NB4-R1 cells infected with P30 expressing or control vector. Thus, we inferred that ATRA sensitivity of NB4-R1 cells was enhanced by restoration of C/EBPα P42. In addition, we used histone deacetylase inhibitor trichostatin (TSA) to restore C/EBPα expression in NB4-R1 cells. Similar enhancement of myeloid differentiation and cell growth arrest were detected. Together, the present study demonstrated that suppression of C/EBPα P42 induced by PI3K/Akt/mTOR inhibition impaired the differentiation and ATRA sensitivity of APL cells. Restoring C/EBPα P42 is an attractive approach for differentiation therapy in ATRA resistant APL." @default.
• W1780521159 created "2016-06-24" @default.
• W1780521159 creator A5026067942 @default.
• W1780521159 creator A5046579620 @default.
• W1780521159 creator A5050803462 @default.
• W1780521159 creator A5065937085 @default.
• W1780521159 creator A5067050151 @default.
• W1780521159 creator A5090013547 @default.
• W1780521159 date "2015-09-14" @default.
• W1780521159 modified "2023-10-16" @default.
• W1780521159 title "Restoration of CCAAT enhancer binding protein α P42 induces myeloid differentiation and overcomes all-trans retinoic acid resistance in human acute promyelocytic leukemia NB4-R1 cells" @default.
• W1780521159 cites W1486948598 @default.
• W1780521159 cites W1602220148 @default.
• W1780521159 cites W1853841955 @default.
• W1780521159 cites W1874102351 @default.
• W1780521159 cites W1912892088 @default.
• W1780521159 cites W1980961069 @default.
• W1780521159 cites W1981044274 @default.
• W1780521159 cites W1988438225 @default.
• W1780521159 cites W1990539158 @default.
• W1780521159 cites W1993111203 @default.
• W1780521159 cites W1997473510 @default.
• W1780521159 cites W1999807375 @default.
• W1780521159 cites W2006699136 @default.
• W1780521159 cites W2007443158 @default.
• W1780521159 cites W2015154759 @default.
• W1780521159 cites W2020649739 @default.
• W1780521159 cites W2034419327 @default.
• W1780521159 cites W2035643006 @default.
• W1780521159 cites W2038345154 @default.
• W1780521159 cites W2042076653 @default.
• W1780521159 cites W2048645509 @default.
• W1780521159 cites W2059132989 @default.
• W1780521159 cites W2063083122 @default.
• W1780521159 cites W2071481953 @default.
• W1780521159 cites W2077872976 @default.
• W1780521159 cites W2081773938 @default.
• W1780521159 cites W2083483484 @default.
• W1780521159 cites W2092648511 @default.
• W1780521159 cites W2096340973 @default.
• W1780521159 cites W2096603155 @default.
• W1780521159 cites W2100088478 @default.
• W1780521159 cites W2105974034 @default.
• W1780521159 cites W2107558453 @default.
• W1780521159 cites W2112641034 @default.
• W1780521159 cites W2113873599 @default.
• W1780521159 cites W2122671437 @default.
• W1780521159 cites W2126955442 @default.
• W1780521159 cites W2131627815 @default.
• W1780521159 cites W2142423276 @default.
• W1780521159 cites W2143075695 @default.
• W1780521159 cites W2150044225 @default.
• W1780521159 cites W2256936668 @default.
• W1780521159 cites W2443384920 @default.
• W1780521159 doi "https://doi.org/10.3892/ijo.2015.3163" @default.
• W1780521159 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4599186" @default.
• W1780521159 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/26397153" @default.
• W1780521159 hasPublicationYear "2015" @default.
• W1780521159 type Work @default.
• W1780521159 sameAs 1780521159 @default.
• W1780521159 citedByCount "7" @default.
• W1780521159 countsByYear W17805211592018 @default.
• W1780521159 countsByYear W17805211592019 @default.
• W1780521159 countsByYear W17805211592020 @default.
• W1780521159 countsByYear W17805211592022 @default.
• W1780521159 countsByYear W17805211592023 @default.
• W1780521159 crossrefType "journal-article" @default.
• W1780521159 hasAuthorship W1780521159A5026067942 @default.
• W1780521159 hasAuthorship W1780521159A5046579620 @default.
• W1780521159 hasAuthorship W1780521159A5050803462 @default.
• W1780521159 hasAuthorship W1780521159A5065937085 @default.
• W1780521159 hasAuthorship W1780521159A5067050151 @default.
• W1780521159 hasAuthorship W1780521159A5090013547 @default.
• W1780521159 hasBestOaLocation W17805211591 @default.
• W1780521159 hasConcept C104317684 @default.
• W1780521159 hasConcept C148738053 @default.
• W1780521159 hasConcept C153911025 @default.
• W1780521159 hasConcept C2776601000 @default.
• W1780521159 hasConcept C2776912716 @default.
• W1780521159 hasConcept C2778729363 @default.
• W1780521159 hasConcept C2779282312 @default.
• W1780521159 hasConcept C2779963572 @default.
• W1780521159 hasConcept C2781121885 @default.
• W1780521159 hasConcept C502942594 @default.
• W1780521159 hasConcept C54355233 @default.
• W1780521159 hasConcept C55493867 @default.
• W1780521159 hasConcept C62112901 @default.
• W1780521159 hasConcept C62478195 @default.
• W1780521159 hasConcept C75217442 @default.
• W1780521159 hasConcept C81885089 @default.
• W1780521159 hasConcept C86554907 @default.
• W1780521159 hasConcept C86803240 @default.
• W1780521159 hasConcept C95444343 @default.
• W1780521159 hasConceptScore W1780521159C104317684 @default.
• W1780521159 hasConceptScore W1780521159C148738053 @default.
• W1780521159 hasConceptScore W1780521159C153911025 @default.
• W1780521159 hasConceptScore W1780521159C2776601000 @default.
• W1780521159 hasConceptScore W1780521159C2776912716 @default.

• next