Matches in SemOpenAlex for { <https://semopenalex.org/work/W1780570168> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W1780570168 abstract "Following crush injury to rat sciatic nerves, a crude fraction of the 150,000 g supernatant can post-translationally incorporate [3H]Arg and [3H]Lys into endogenous proteins in amounts approximately 10 times uninjured control nerves. These increases occur in the proximal nerve stump within 2 h of injury and 2 weeks later in a distal segment of nerve containing the tips of the regenerating axons. In the present experiments, the endogenous nerve proteins modified by Arg or Lys in these nerve segments have been identified using two-dimensional polyacrylamide gel electrophoresis. The fraction used to assay for protein modification, the void volume of a Sephacryl S-300 column, was found to contain only a few proteins visible by Coomassie blue staining, one of which is likely to be albumin (68 kDa, pI 6.4). While this protein was modified by both Arg and Lys, the majority of label was found in areas not showing Coomassie blue staining. This indicates that of the many potential targets of post-translational arginylation and lysylation, most are proteins of relatively low abundance. A variety of proteins were modified by Arg or Lys alone while others were modified by both Arg and Lys. A high molecular weight protein (175 kDa, pI 9.0) was modified only by Lys and only at 2 h post crush. Of a variety of modified proteins of approximately 17 kDa one (pI 6.3) was modified by both Arg and Lys and at both time points, while another (pI 9.0) was modified at both time points, but only by Lys. The results show that Arg and Lys can be added post-translationally to a large number of low abundance, soluble sciatic nerve proteins, and that some of those proteins are modified only by Arg or Lys while others are modified by both Arg and Lys. Also, the modification of certain proteins appears to be associated specifically with the immediate response of a nerve to injury (e.g. 88 kDa, pI 7.1) while others are associated with the regenerative period (e.g. 56 kDa, pI 7.4)." @default.
- W1780570168 created "2016-06-24" @default.
- W1780570168 creator A5070316954 @default.
- W1780570168 creator A5078643264 @default.
- W1780570168 creator A5087889403 @default.
- W1780570168 date "1990-01-01" @default.
- W1780570168 modified "2023-09-26" @default.
- W1780570168 title "Post-translational modification of proteins by arginine and lysine following crush injury and during regeneration of rat sciatic nerves" @default.
- W1780570168 doi "https://doi.org/10.3233/rnn-1990-2201" @default.
- W1780570168 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21551586" @default.
- W1780570168 hasPublicationYear "1990" @default.
- W1780570168 type Work @default.
- W1780570168 sameAs 1780570168 @default.
- W1780570168 citedByCount "7" @default.
- W1780570168 countsByYear W17805701682016 @default.
- W1780570168 countsByYear W17805701682020 @default.
- W1780570168 crossrefType "journal-article" @default.
- W1780570168 hasAuthorship W1780570168A5070316954 @default.
- W1780570168 hasAuthorship W1780570168A5078643264 @default.
- W1780570168 hasAuthorship W1780570168A5087889403 @default.
- W1780570168 hasConcept C101660505 @default.
- W1780570168 hasConcept C105702510 @default.
- W1780570168 hasConcept C116084860 @default.
- W1780570168 hasConcept C153911025 @default.
- W1780570168 hasConcept C181199279 @default.
- W1780570168 hasConcept C185592680 @default.
- W1780570168 hasConcept C2776016237 @default.
- W1780570168 hasConcept C2777468819 @default.
- W1780570168 hasConcept C2780761556 @default.
- W1780570168 hasConcept C2781149210 @default.
- W1780570168 hasConcept C515207424 @default.
- W1780570168 hasConcept C54355233 @default.
- W1780570168 hasConcept C55493867 @default.
- W1780570168 hasConcept C74864618 @default.
- W1780570168 hasConcept C86803240 @default.
- W1780570168 hasConceptScore W1780570168C101660505 @default.
- W1780570168 hasConceptScore W1780570168C105702510 @default.
- W1780570168 hasConceptScore W1780570168C116084860 @default.
- W1780570168 hasConceptScore W1780570168C153911025 @default.
- W1780570168 hasConceptScore W1780570168C181199279 @default.
- W1780570168 hasConceptScore W1780570168C185592680 @default.
- W1780570168 hasConceptScore W1780570168C2776016237 @default.
- W1780570168 hasConceptScore W1780570168C2777468819 @default.
- W1780570168 hasConceptScore W1780570168C2780761556 @default.
- W1780570168 hasConceptScore W1780570168C2781149210 @default.
- W1780570168 hasConceptScore W1780570168C515207424 @default.
- W1780570168 hasConceptScore W1780570168C54355233 @default.
- W1780570168 hasConceptScore W1780570168C55493867 @default.
- W1780570168 hasConceptScore W1780570168C74864618 @default.
- W1780570168 hasConceptScore W1780570168C86803240 @default.
- W1780570168 hasLocation W17805701681 @default.
- W1780570168 hasLocation W17805701682 @default.
- W1780570168 hasOpenAccess W1780570168 @default.
- W1780570168 hasPrimaryLocation W17805701681 @default.
- W1780570168 hasRelatedWork W1605953390 @default.
- W1780570168 hasRelatedWork W1694722751 @default.
- W1780570168 hasRelatedWork W1783614608 @default.
- W1780570168 hasRelatedWork W1964680006 @default.
- W1780570168 hasRelatedWork W1968656266 @default.
- W1780570168 hasRelatedWork W1971521731 @default.
- W1780570168 hasRelatedWork W1978495037 @default.
- W1780570168 hasRelatedWork W2011186002 @default.
- W1780570168 hasRelatedWork W2011876166 @default.
- W1780570168 hasRelatedWork W2027834618 @default.
- W1780570168 hasRelatedWork W2048215168 @default.
- W1780570168 hasRelatedWork W2051558237 @default.
- W1780570168 hasRelatedWork W2060546155 @default.
- W1780570168 hasRelatedWork W2071056869 @default.
- W1780570168 hasRelatedWork W2075330831 @default.
- W1780570168 hasRelatedWork W2085127800 @default.
- W1780570168 hasRelatedWork W2157891357 @default.
- W1780570168 hasRelatedWork W2395960644 @default.
- W1780570168 hasRelatedWork W2186441260 @default.
- W1780570168 hasRelatedWork W2214093755 @default.
- W1780570168 isParatext "false" @default.
- W1780570168 isRetracted "false" @default.
- W1780570168 magId "1780570168" @default.
- W1780570168 workType "article" @default.