Matches in SemOpenAlex for { <https://semopenalex.org/work/W17810837> ?p ?o ?g. }
- W17810837 abstract "The concept of de novo metabolic engineering through novel synthetic pathways offers new directions for multi-step enzymatic synthesis of complex molecules. This has been complemented by recent progress in performing enzymatic reactions using immobilised enzyme microreactors (IEMR). This work is concerned with the construction of de novo designed enzyme pathways in a microreactor synthesising a chiral molecule. An interesting compound, commonly used as the building block in several pharmaceutical syntheses, is a single diastereoisomer of 2-amino-1,3,4-butanetriol (ABT). This chiral amino alcohol can be synthesised from simple achiral substrates using two enzymes, transketolase (TK) and ω-transaminase (TAm). This project involves the design and the development of an IEMR using His6-tagged TK and TAm immobilised onto Ni-NTA agarose beads and packed into tubes to enable multi-step enzyme reactions. The IEMR was first characterised based on the operational and storage stability. Furthermore, kinetic parameters of both enzymes were determined using single IEMRs evaluated by a kinetic model developed for packed bed reactors. For the multi-step enzyme reaction, two model systems were investigated. The first model investigated was the dual TK (pQR 791)-TAm (pQR 801) reaction. With initial 60 mM (HPA and GA each) and 6 mM (MBA) substrate concentration mixture, the coupled reaction reached approximately 83% conversion in 20 minutes at the lowest flow rate. On the other hand, the second model reaction comprises of three sequential enzyme reaction, TAm (pQR 1021)-TK (pQR 791)-TAm (pQR 1021). A 6% yield of ABT was produced from initial substrate mixture of 100 mM serine and 40 mM GA at flow rate of 0.5 μL/min. Further considerations to improve the system would allow for better yield of the target product and potentially make this IEMR system a powerful tool for construction and evaluation of de novo pathways as well as for rapid determination of various enzymes kinetics." @default.
- W17810837 created "2016-06-24" @default.
- W17810837 creator A5017828164 @default.
- W17810837 date "2014-01-28" @default.
- W17810837 modified "2023-09-24" @default.
- W17810837 title "In vitro metabolic pathway construction in an immobilised enzyme microreactor (IEMR)" @default.
- W17810837 cites W1493787054 @default.
- W17810837 cites W1501816035 @default.
- W17810837 cites W1530826051 @default.
- W17810837 cites W1577450737 @default.
- W17810837 cites W1594964467 @default.
- W17810837 cites W1652052270 @default.
- W17810837 cites W1707734422 @default.
- W17810837 cites W1866051727 @default.
- W17810837 cites W18982056 @default.
- W17810837 cites W1909235131 @default.
- W17810837 cites W1947975057 @default.
- W17810837 cites W1964151481 @default.
- W17810837 cites W1964554326 @default.
- W17810837 cites W1964938953 @default.
- W17810837 cites W1966373074 @default.
- W17810837 cites W1967590589 @default.
- W17810837 cites W1968042342 @default.
- W17810837 cites W1968055469 @default.
- W17810837 cites W1969968380 @default.
- W17810837 cites W1972140003 @default.
- W17810837 cites W1972873278 @default.
- W17810837 cites W1972907321 @default.
- W17810837 cites W1975625965 @default.
- W17810837 cites W1975687263 @default.
- W17810837 cites W1976773992 @default.
- W17810837 cites W1977236778 @default.
- W17810837 cites W1977477798 @default.
- W17810837 cites W1977780349 @default.
- W17810837 cites W1978106567 @default.
- W17810837 cites W1978953376 @default.
- W17810837 cites W1979338300 @default.
- W17810837 cites W1980271377 @default.
- W17810837 cites W1981199746 @default.
- W17810837 cites W1983045936 @default.
- W17810837 cites W1983607795 @default.
- W17810837 cites W1983641626 @default.
- W17810837 cites W1984406292 @default.
- W17810837 cites W1984982134 @default.
- W17810837 cites W1985830439 @default.
- W17810837 cites W1986972481 @default.
- W17810837 cites W1987362398 @default.
- W17810837 cites W1987908607 @default.
- W17810837 cites W1988335682 @default.
- W17810837 cites W1988630698 @default.
- W17810837 cites W1988654953 @default.
- W17810837 cites W1988752605 @default.
- W17810837 cites W1989371246 @default.
- W17810837 cites W1990302863 @default.
- W17810837 cites W1990676461 @default.
- W17810837 cites W1991040598 @default.
- W17810837 cites W1991498111 @default.
- W17810837 cites W1992060322 @default.
- W17810837 cites W1992106162 @default.
- W17810837 cites W1993143817 @default.
- W17810837 cites W1993266050 @default.
- W17810837 cites W1994846754 @default.
- W17810837 cites W1994926929 @default.
- W17810837 cites W1995502565 @default.
- W17810837 cites W1995809651 @default.
- W17810837 cites W1996284845 @default.
- W17810837 cites W1996566094 @default.
- W17810837 cites W1998641515 @default.
- W17810837 cites W1998966759 @default.
- W17810837 cites W1999617108 @default.
- W17810837 cites W2002404487 @default.
- W17810837 cites W2002997951 @default.
- W17810837 cites W2005224598 @default.
- W17810837 cites W2005466967 @default.
- W17810837 cites W2006473130 @default.
- W17810837 cites W2006727769 @default.
- W17810837 cites W2007363842 @default.
- W17810837 cites W2007790651 @default.
- W17810837 cites W2008669956 @default.
- W17810837 cites W2011176120 @default.
- W17810837 cites W2014066827 @default.
- W17810837 cites W2014811157 @default.
- W17810837 cites W2015569232 @default.
- W17810837 cites W2016594917 @default.
- W17810837 cites W2017777806 @default.
- W17810837 cites W2019018073 @default.
- W17810837 cites W2019472659 @default.
- W17810837 cites W2019915212 @default.
- W17810837 cites W2021457845 @default.
- W17810837 cites W2022366177 @default.
- W17810837 cites W2024286012 @default.
- W17810837 cites W2025467097 @default.
- W17810837 cites W2026048837 @default.
- W17810837 cites W2026104248 @default.
- W17810837 cites W2027502160 @default.
- W17810837 cites W2028666920 @default.
- W17810837 cites W2028670901 @default.
- W17810837 cites W2029024859 @default.
- W17810837 cites W2029438144 @default.
- W17810837 cites W2030560807 @default.