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- W178142720 abstract "Endogenous glucose synthesis is crucial to the maintenance of adequate glucose supplies for the body. The conversions of glycine to serine and of serine and alanine to pyruvate are pyridoxal 5′-phosphate (PLP)-dependent, leading to a metabolic intermediate which can be used for gluconeogenesis. This study aims to assess the impact of vitamin B-6 deficiency on gluconeogenesis from amino acid precursors in a human hepatoma cell line. HepG2 cells were cultured in vitamin B-6 replete (2000 nM pyridoxal) or vitamin B-6-deficient Dulbecco's minimal essential medium containing 10, 20, or 50 nM pyridoxal. At steady-state, intracellular PLP levels were 29.1, 37.6, 42.5, and 57.2 pmol/mg protein, respectively. Cells were cultured for 24h in media enriched with a [U-13C3]serine tracer, and enrichment of intra- and extracellular serine, glycine, and glucose measured by GC/MS. Maximum glucose enrichment occurred as the singly-labeled species at 4h. Investigation is underway evaluate the effect of vitamin B-6 status on gluconeogenic rate. Grant Funding Source: UFL" @default.
- W178142720 created "2016-06-24" @default.
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- W178142720 date "2010-04-01" @default.
- W178142720 modified "2023-09-27" @default.
- W178142720 title "Impact of vitamin B‐6 availability on gluconeogenesis from amino acid precursors in human hepatoma (HepG2) cells" @default.
- W178142720 doi "https://doi.org/10.1096/fasebj.24.1_supplement.915.4" @default.
- W178142720 hasPublicationYear "2010" @default.
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