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- W1783313194 abstract "The most important function of the human skin is the protection of the individual from the external pathogens and other harmful influences. In our skin a specialized microbial community exists, called the skin microbiome, suggested to have direct effects on various molecular and cellular properties of the human cells.Our aim was to analyze whether the Propionibacterium acnes (P. acnes) bacterium, a member of the microbiome has any effect on the barrier properties of the skin. For that, HPV-KER cells (human immortalized keratinocytes) were treated with various P.acnes strains (889, 6609, ATCC 11828) in different doses, and the barrier changes were analyzed in real time using the xCELLIgence system.Our results suggests that the P. acnes induced dose-dependent changes, where the effect of the pathogenic 889 and ATCC 11828 strains appeared more robust.We also analyzed the effect of the bacterium on the mRNA expression changes of tight junction proteins claudin 1, 2, 4 (CLDN1, 2, 4), ocludin1 (OCL1) and ZO1 in confluent HPV-KER cultures using real time RT-PCR. Except for CLDN2 and 4 all of the analyzed genes were present at the mRNA level in these cells. The expression of CLDN1 decreased, whereas ZO1 mRNA levels increased in response to the bacterial treatment.P. acnes can also induce innate immune and inflammatory events in keratinocytes. Thus, we studied if the TNF pro-inflammatory cytokine treatment (1, 5, 10 ng/ml) can modify the barrier properties of the HPV-KER cells. We found that TNFα, and thus inflammatory conditions may loosen the epidermal barrier, possibly causing the easier penetration of keratinocyte- as well as bacterial-derived factors to deeper tissue compartments.These findings suggest that the skin microbiome can modulate the barrier properties of the human skin, and these event may have important role in the pathogenesis of different skin diseases." @default.
- W1783313194 created "2016-06-24" @default.
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- W1783313194 date "2014-01-01" @default.
- W1783313194 modified "2023-09-22" @default.
- W1783313194 title "The effect of the skin microbiome on the barrier properties of in vitro cultured keratinocytes" @default.
- W1783313194 hasPublicationYear "2014" @default.
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