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- W1783339571 abstract "The sensitivity, selectivity, and ease of use of nucleic acid probes and the availability of efficient protocols for the isolation and cloning of specific DNA sequences have led to the development of a wide selection of probes for biomedical and clinical applications. In the basic hybridization protocol, a labeled nucleic acid probe is annealed to a complementary DNA or RNA target sequence, which either is in solution or is immobilized on an inert support. The labeled nucleic acid probe is used to determine the presence or absence of the target sequence in the reaction mixture. An effective nonradioactive detection system should recognize the annealed nucleic acid probe with a degree of precision comparable to that obtained in the primary hybridization reaction. The presence of either a free amine or biotin may also be ascertained by chemical analysis. Compounds containing primary amines give positive color development when spotted on thin layer chromatography (TLC) plates and sprayed with ninhydrin. The first approach uses iminobiotin, an analog of biotin in which the ureido group has been replaced with a guanido group. Iminobiotin binds to avidin with a binding constant that increases with increasing pH and nonprotonated iminobiotin binds efficiently to avidin." @default.
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- W1783339571 date "1990-01-01" @default.
- W1783339571 modified "2023-09-25" @default.
- W1783339571 title "[66] Biotinylated nucleotides for labeling and detecting DNA" @default.
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- W1783339571 doi "https://doi.org/10.1016/0076-6879(90)84320-g" @default.
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