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- W178366102 abstract "Agrobacterium strains containing nopaline-type Ti plasmids are able to transfer DNA to maize whereas strains containing octopine-type Ti plasmids cannot. In this study the genetic factors controlling the Agrobacterium strain-specificity of DNA transfer to maize have been investigated. “Agroinfection” of maize streak virus (MSV) DNA provided a “reporter” system for successful transfer. Mutation of the strain-specific vir loci showed that only the octopine-specific virF locus affected DNA transfer. However, the increased efficiency of DNA transfer obtained using these mutants was insufficient to account for the strain specificity. Introduction of fragments of the octopine Ti plasmid into nopaline strains did not inhibit DNA transfer whereas the presence of a 10.6kb fragment of the nopaline Ti plasmid in octopine strains promoted efficient transfer. Efficient agroinfection was also obtained using an octopine virA mutant strain which constitutively expresses the virA gene; since the complementing fragment contained the virA locus these data suggest that the inability of octopine strains to promote DNA transfer is due to a lack of vir gene induction.KeywordsAgrobacterium StrainTrans ZeatinMaize Streak VirusRice Tungro Bacilliform VirusvirA LocusThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves." @default.
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- W178366102 date "1993-01-01" @default.
- W178366102 modified "2023-09-27" @default.
- W178366102 title "Identification of Genetic Factors Controlling the Ability of Agrobacterium to Transfer DNA to Maize" @default.
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- W178366102 doi "https://doi.org/10.1007/978-94-017-0651-3_7" @default.
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